Li S Y
Institute of Basic Medical Sciences, Beijing.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1990 Jun;12(3):227-30.
With dot blot and cytoplasmic hybridization techniques we found that the c-Ha-ras, c-Ki-ras, c-N-ras, c-myc and c-fos oncogenes were over-expressed in human hepatocellular carcinoma cell line BEL 7402 cells. The mRNA expression of c-Ha-ras, c-Ki-ras, c-N-ras and c-myc oncogenes could be inhibited by 2 mmol/L sodium butyrate treatment, but this had no effect on the expression of c-fos. However, the mRNA expression of c-Ha-ras, c-N-Ras and c-myc oncogenes was enhanced by 4 mmol/L sodium butyrate treatment, while the expression of c-Ki-ras and c-fos remained unchanged. No significant effect on the expression of carbamyl phosphate synthetase I, a tissue-specific enzyme associated with the differentiation of liver cells, was observed by 2 mmol/L or 4 mmol/L sodium butyrate treatment of the hepatoma cells.
通过斑点印迹和细胞质杂交技术，我们发现c-Ha-ras、c-Ki-ras、c-N-ras、c-myc和c-fos癌基因在人肝癌细胞系BEL 7402细胞中过表达。2 mmol/L丁酸钠处理可抑制c-Ha-ras、c-Ki-ras、c-N-ras和c-myc癌基因的mRNA表达，但对c-fos的表达无影响。然而，4 mmol/L丁酸钠处理可增强c-Ha-ras、c-N-Ras和c-myc癌基因的mRNA表达，而c-Ki-ras和c-fos的表达保持不变。用2 mmol/L或4 mmol/L丁酸钠处理肝癌细胞，未观察到对氨甲酰磷酸合成酶I（一种与肝细胞分化相关的组织特异性酶）表达的显著影响。
