Gibert I, Barbé J, Casadesús J
Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, Spain.
J Gen Microbiol. 1990 Dec;136(12):2555-60. doi: 10.1099/00221287-136-12-2555.
Two DNA probes for the detection of insertion sequence IS200 by either Southern blotting or colony hybridization were constructed. One of the probes is a 300 bp EcoRI-HindIII fragment of IS200 cloned onto pBluescript KS(+); the other is a tail-to-tail dimer of the same fragment cloned onto pUC19. A survey of the presence of IS200 among enteric bacteria revealed that more than 90% of the pathogenic or food-poisoning isolates of Salmonella spp. examined contained one or more copies of insertion sequence IS200, with the exception of the subgenus I serovar S. agona in which IS200 is not found. Although insertion sequence IS200 was first considered a Salmonella-specific element, it also exists in many isolates of Shigella sonnei and Shigella flexneri, but not in Shigella dysenteriae.
构建了两种用于通过Southern印迹法或菌落杂交法检测插入序列IS200的DNA探针。其中一种探针是克隆到pBluescript KS(+)上的IS200的300 bp EcoRI-HindIII片段；另一种是克隆到pUC19上的同一片段的尾对尾二聚体。对肠道细菌中IS200存在情况的调查显示，除了未发现IS200的I亚种血清型阿贡纳沙门氏菌外，超过90%检测的致病性或食物中毒性沙门氏菌分离株含有一个或多个插入序列IS200拷贝。尽管插入序列IS200最初被认为是沙门氏菌特异性元件，但它也存在于许多宋内志贺氏菌和福氏志贺氏菌分离株中，而痢疾志贺氏菌中不存在。
