Beisel Christian, Paro Renato
Department Biosystems Science and Engineering, ETH Zurich, Mattenstrasse 26, CH-4058 Basel, Switzerland.
Biol Chem. 2009 Nov;390(11):1139-44. doi: 10.1515/BC.2009.116.
Transcription factor regulation of gene expression and chromatin-controlled epigenetic memory systems are closely cooperating in establishing the pluripotent state of embryonic stem (ES) cells and maintaining cell fate decisions throughout development of an organism. A thorough understanding of the regulatory transcriptional circuitry that rules the underlying plastic yet heritable gene expression programs in ES cells is of great importance. With the advent of next-generation sequencing technologies facilitating the quantitative assessment of functional genomics assays it is now feasible to interrogate transcription networks at a genome-wide scale. Here, we discuss the application of next-generation sequencing in elucidating the molecular mechanisms underlying ES cell function.
转录因子对基因表达的调控以及染色质控制的表观遗传记忆系统，在建立胚胎干细胞（ES细胞）的多能状态以及在生物体整个发育过程中维持细胞命运决定方面密切协作。深入了解调控ES细胞中潜在的可塑性但可遗传的基因表达程序的转录调控电路至关重要。随着促进功能基因组学检测定量评估的新一代测序技术的出现，现在在全基因组范围内研究转录网络已成为可能。在此，我们讨论新一代测序在阐明ES细胞功能潜在分子机制方面的应用。
