Ku Seung-Jun, Chang Young-Il, Chae Chang-Hoon, Kim Seong-Gon, Park Young-Wook, Jung Youn-Kwan, Choi Je-Yong
Department of Orthodontics, Seoul National University, Seoul 110-749, Korea.
BMB Rep. 2009 Jul 31;42(7):427-32. doi: 10.5483/bmbrep.2009.42.7.427.
Orthodontic tooth movement results from the combinational process of both bone resorption and formation in the compressive and tension sides, respectively. However, the genes responsible for new bone formation in tension sides have not been determined. In this study, we used DNA microarray and real-time RT-PCR to identify genes in human periodontal ligament (PDL) cells that undergo significant changes in expression in response to static tensional forces (2 or 12 hours). The genes found were alkaline phospatase (ALP), matrix metalloproteinases (MMPs), vascular endothelial growth factor (VEGF), and several collagen genes. Furthermore, an ELISA evaluating the expression of VEGF, type IV collagen and MMP-2 found levels significantly increased after 24 and 72 hours (P 0.05). ALP activity was also increased after 24 hours (P 0.05). Collectively, we found the genes up-regulated in our study by the static tensional force are related to osteogenic processes such as matrix synthesis and angiogenesis.
正畸牙齿移动分别源于压缩侧和张力侧骨吸收与形成的联合过程。然而，负责张力侧新骨形成的基因尚未确定。在本研究中，我们使用DNA微阵列和实时逆转录聚合酶链反应来鉴定人牙周膜（PDL）细胞中因静态张力（2或12小时）而发生显著表达变化的基因。发现的基因有碱性磷酸酶（ALP）、基质金属蛋白酶（MMPs）、血管内皮生长因子（VEGF）和几个胶原基因。此外，一项评估VEGF、IV型胶原和MMP-2表达的酶联免疫吸附测定发现，24和72小时后水平显著升高（P<0.05）。24小时后ALP活性也升高（P<0.05）。总体而言，我们发现在本研究中因静态张力而上调的基因与诸如基质合成和血管生成等成骨过程相关。
