Jang Hyun, Kim Hyo-Seung, Moon Seung-Cheol, Lee Young-Rae, Yu Kang-Yeoul, Lee Byeong-Kil, Youn Hyun Zo, Jeong Young-Ju, Kim Byeong-Soo, Lee Sung-Ho, Kim Jong-Suk
1R&D Center, Komipharm Co., Shihung, Korea.
BMB Rep. 2009 Jul 31;42(7):462-6. doi: 10.5483/bmbrep.2009.42.7.462.
Lipopolysaccharide (LPS), found in the outer membrane of Gram negative bacteria, only exerts its toxic effects when in free form. LPS has three major parts, lipid A, the toxic component, along with a core polysaccharide and O-specific polysaccharide. LPS monomers are known to have molecular masses between 10 to 30 kDa. Under physiological conditions, LPS exists in equilibrium between monomer and vesicle forms. LPS removal by 100 kDa ultrafiltration was more efficient (99.6% of LPS removed) with a low concentration of protein (2.0 mg/ml) compared to a high concentration (20.1 mg/ml). In the presence of different detergents (0.5% Tween 20, 1.0% taurodeoxycholate and 1.0% Triton X-100), LPS removal was more efficient at low protein concentrations (2.0 mg/ml) compared to high protein concentrations (20.1 mg/ml).
脂多糖（LPS）存在于革兰氏阴性菌的外膜中，只有以游离形式存在时才会发挥其毒性作用。LPS有三个主要部分，即毒性成分脂质A，以及核心多糖和O-特异性多糖。已知LPS单体的分子量在10至30 kDa之间。在生理条件下，LPS以单体和囊泡形式处于平衡状态。与高浓度蛋白质（20.1 mg/ml）相比，在低浓度蛋白质（2.0 mg/ml）时，通过100 kDa超滤去除LPS的效率更高（去除了99.6%的LPS）。在存在不同去污剂（0.5%吐温20、1.0%牛磺脱氧胆酸盐和1.0% Triton X-100）的情况下，与高蛋白质浓度（20.1 mg/ml）相比，在低蛋白质浓度（2.0 mg/ml）时LPS的去除效率更高。
