使用达科细胞公司试剂套件在贝克曼免疫分析仪上进行β2微球蛋白检测的性能特征。
Performance characteristics of beta-2-microglobulin measurements on a Beckman Immage analyzer with the DakoCytomation reagent kit.
作者信息
De Barun K, Jimenez Ernest, De Soumo
机构信息
Department of Pathology, University of Arizona Health Sciences Center, Tucson, AZ 85724-5059, USA.
出版信息
Clin Biochem. 2009 Oct;42(15):1577-81. doi: 10.1016/j.clinbiochem.2009.06.030. Epub 2009 Jul 28.
OBJECTIVE
Evaluation of serum beta-2-microglobulin (beta2M) in an automated analyzer.
DESIGN AND METHODS
The DakoCytomation beta2M kit is an antibody based reagent intended for quantitative determination of beta2M in serum and plasma by rate nephelometry.
RESULTS
The limit of blank is 0.16 mg/L. The method is linear up to 17.9 mg/L. The imprecision ranged from 2.1% to 7.9% at the concentrations of 1.77 and 7.19 mg/L, respectively. Method comparison yielded slope=1.009, r=0.998. No interference was observed from hemolytic or icteric specimens. Reference interval of a healthy population was 1.13 mg/L to 3.04 mg/L.
CONCLUSION
The DakoCytomation reagent is acceptable to measure serum beta2M.
目的
在自动分析仪中评估血清β2-微球蛋白（β2M）。
设计与方法
DakoCytomation β2M试剂盒是一种基于抗体的试剂，旨在通过速率散射比浊法对血清和血浆中的β2M进行定量测定。
结果
空白限为0.16mg/L。该方法在高达17.9mg/L时呈线性。在浓度分别为1.77mg/L和7.19mg/L时，不精密度范围为2.1%至7.9%。方法比较得出斜率=1.009，r=0.998。未观察到溶血或黄疸样本的干扰。健康人群的参考区间为1.13mg/L至3.04mg/L。
结论
DakoCytomation试剂可用于测定血清β2M。
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