Shionoya Kiseko, Datiche Frédérique
Centre Européen des Sciences du Goût, CNRS UMR 5170, 15 rue Hugues Picardet, 21000 Dijon, France.
Neurobiol Learn Mem. 2009 Nov;92(4):590-6. doi: 10.1016/j.nlm.2009.07.008. Epub 2009 Jul 28.
Memory reorganization as a time-dependent process can be investigated using various learning tasks such as the taste-potentiated odor aversion (TPOA). In this paradigm rats acquire a strong aversion to an olfactory cue presented simultaneously with a gustatory cue. Together these cues are paired with a delayed visceral illness. The basolateral amygdaloid nucleus (BLA) plays a key role in TPOA acquisition but its involvement in retrieval remains unclear. We investigated the involvement of the BLA in either recent or remote retrieval of TPOA. In each case, the number of licks observed in response to the presentation of either the odor or the taste was used to assess retrieval. Before the retrieval test, rats received a bilateral infusion of lidocaine to inactivate the BLA. We observed that both recent and remote TPOA retrieval tests induced by the odor presentation were disrupted in the lidocaine-injected rats. By contrast, the BLA inactivation had no effect upon the aversion towards the taste cue regardless of the time of retrieval. The present study provides evidence that BLA functioning is necessary for retrieval of aversive odor memory, even with a long post-acquisition delay.
作为一个时间依赖性过程的记忆重组，可以通过各种学习任务来研究，比如味觉增强气味厌恶（TPOA）。在这个范式中，大鼠会对与味觉线索同时呈现的嗅觉线索产生强烈的厌恶。这些线索一起与延迟的内脏疾病配对。基底外侧杏仁核（BLA）在TPOA习得中起关键作用，但其在提取过程中的作用仍不清楚。我们研究了BLA在TPOA近期或远期提取中的作用。在每种情况下，通过观察大鼠对气味或味道呈现的舔舐次数来评估提取情况。在提取测试前，给大鼠双侧注射利多卡因以使BLA失活。我们观察到，在注射利多卡因的大鼠中，由气味呈现诱导的近期和远期TPOA提取测试均受到干扰。相比之下，无论提取时间如何，BLA失活对味觉线索的厌恶没有影响。本研究提供了证据，表明即使在习得后有很长的延迟，BLA的功能对于厌恶气味记忆的提取也是必要的。
