Liu Jia, Huang Xiang-Feng, Lu Li-Jun, Xu Jing-Cheng, Wen Yue, Yang Dian-Hai, Zhou Qi
College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai, 200092, China.
Bioresour Technol. 2009 Dec;100(24):6481-7. doi: 10.1016/j.biortech.2009.07.006. Epub 2009 Jul 29.
In order to lower the production cost, waste frying oils were used in the biosynthesis of demulsifier by Dietzia sp. S-JS-1, which was isolated from petroleum contaminated soil. After 7 days of cultivation, the biomass concentration of the most suitable waste frying oil (WFO II) culture reached 3.78 g/L, which was 2.4 times the concentration of paraffin culture. The biodemulsifier produced with WFO II culture broke the emulsions more efficiently than that produced with paraffin culture, given the same volume ratio of carbon source in the culture medium and the same cultivation conditions. It achieved 88.3% of oil separation ratio in W/O emulsion and 76.4% of water separation ratio in O/W emulsion within 5 h. With the aid of thin layer chromatography (TLC) and Fourier transform infrared (FTIR) spectrometry, biodemulsifiers produced from both paraffin and WFO II were identified as a mixture of lipopeptide homologues. The subtle variation in the distribution of these homologues and high biomass concentration of WFO II cultures may account for the afore-mentioned good demulsification performance.
为了降低生产成本，Dietzia sp. S-JS-1（从石油污染土壤中分离得到）利用废煎炸油进行破乳剂的生物合成。培养7天后，最适宜的废煎炸油（WFO II）培养物的生物量浓度达到3.78 g/L，是石蜡培养物浓度的2.4倍。在培养基中碳源体积比相同且培养条件相同的情况下，用WFO II培养物生产的生物破乳剂比用石蜡培养物生产的生物破乳剂破乳效率更高。在5小时内，它在W/O乳液中的油分离率达到88.3%，在O/W乳液中的水分离率达到76.4%。借助薄层色谱（TLC）和傅里叶变换红外（FTIR）光谱，由石蜡和WFO II生产的生物破乳剂均被鉴定为脂肽同系物的混合物。这些同系物分布的细微差异以及WFO II培养物的高生物量浓度可能是上述良好破乳性能的原因。
