Lagoudianakis Emmanuel E, Kataki Agapi, Manouras Andreas, Memos Nikolaos, Papadima Artemisia, Derventzi Anastasia, Zografos George, Papadopoulos Stefanos, Katergiannakis Vaggelogiannis, Konstadoulakis Manoussos M
First Department of Propaedeutic Surgery, Hippocrateion Hospital, Athens Medical School, Athens, Greece.
J Surg Res. 2009 Aug;155(2):183-90. doi: 10.1016/j.jss.2007.10.013.
The detection of epithelial-specific mRNA correlates well with the presence of cancer cells in the peripheral blood and provides a rational explanation for subsequent metastasis.
Forty-two, patients with colorectal cancer and 14 controls were included in our study. Peripheral blood samples were acquired at 24 h before and 48 h after laparotomy. Tissue samples were also acquired from the primary lesion. All samples were examined for the expression profile of CEA, CK20, and TEM-8.
Tissue samples expressed CEA in every specimen, CK20 in 30, and TEM-8 in 41. CEA and CK20 were not identified in the control blood samples while TEM-8 was detected in 4. CEA was detected in 17, CK20 in 28 and TEM-8 in 23, of the preoperative blood samples. CEA mRNA expression in preoperative blood sample and TNM stage were found independently associated with increased tumor size. Positive CEA, CK20, and TEM-8 signals were found in 25, 25, and 23 of the postoperative blood samples respectively.
CK20 and CEA are significantly more frequently detected in colon cancer patients than in healthy controls and can serve as markers. Cancer cell mRNA is commonly detected in the preoperative and postoperative peripheral blood samples. Tumor size was independently associated with the preoperative detection of CEA mRNA. Although TEM-8 mRNA detection in the peripheral blood showed no specificity for cancer patients or correlation with clinical stage, identification and validation of genes and proteins implicated in metastatic process needs to be further investigated.
上皮特异性mRNA的检测与外周血中癌细胞的存在密切相关，并为随后的转移提供了合理的解释。
本研究纳入了42例结直肠癌患者和14例对照。在剖腹手术前24小时和术后48小时采集外周血样本。还从原发灶采集组织样本。所有样本均检测CEA、CK20和TEM-8的表达谱。
组织样本中每个标本均表达CEA，30个表达CK20，41个表达TEM-8。对照血样本中未检测到CEA和CK20，而4个检测到TEM-8。术前血样本中，17个检测到CEA，28个检测到CK20，23个检测到TEM-8。术前血样本中CEA mRNA表达和TNM分期与肿瘤大小增加独立相关。术后血样本中分别有25个、25个和23个检测到CEA、CK20和TEM-8阳性信号。
与健康对照相比，结直肠癌患者中CK20和CEA的检测频率显著更高，可作为标志物。术前和术后外周血样本中普遍检测到癌细胞mRNA。肿瘤大小与术前CEA mRNA检测独立相关。虽然外周血中TEM-8 mRNA检测对癌症患者无特异性，也与临床分期无关，但参与转移过程的基因和蛋白质的鉴定与验证仍需进一步研究。
