Pan Chao-mei, Li Wei, He Hong, Deng Wang-qiu, Li Tai-hui, Xu Hong-hua
College of Pharmacy, Guangzhou University of Traditional Chinese Medicine.
Zhongguo Zhong Yao Za Zhi. 2006 May;31(9):723-6.
To study the genetic polymorphism and intraspecific genetic differentiation of different populations of Pogostemon cablin, and find out the effective method to distinguish DNA fingerprint of different populations of P. cablin.
Five plant populations of P. cablin were analyzed by RAPD markers. PopGen 32 software for clustering analysis and calculating. Fourteen of the 80 random primers were tested to possess the stronger detecting effect of polymorphous character.
A total of 84 bands was amplified by the 10 primers, among them 17 bands were monomorphic. 67 of them were polymorphic. The results indicated that the genetic variations existed within the different plant populations of the same species.
It is feasible by RAPD technique with specifically primer to analyze the genetic diversity and identify 5 plant populations of P. cablin. RAPD technique has provided a new path for identification and classification of P. cablin genetic germplasm.
研究广藿香不同居群的遗传多态性及种内遗传分化，探寻区分广藿香不同居群DNA指纹图谱的有效方法。
采用RAPD标记对5个广藿香植物居群进行分析，运用PopGen 32软件进行聚类分析和计算。在80条随机引物中筛选出14条对多态性具有较强检测效果的引物。
10条引物共扩增出84条带，其中17条为单态带，67条为多态带。结果表明同一物种不同植物居群间存在遗传变异。
利用RAPD技术结合特异性引物分析广藿香的遗传多样性并鉴别5个广藿香植物居群是可行的。RAPD技术为广藿香遗传种质的鉴定和分类提供了一条新途径。
