Liu Yang, Zahida Parveen, Deng Yu-Lin, Zhang Jing-Hua, Dai Rong-Ji
Beijing Center for Physical and Chemical Analysis, Beijing 100089, China.
Zhong Yao Cai. 2009 Apr;32(4):518-20.
To study the flavonoids compounds from the effective fraction of Thesium chinese in anti-inflammation.
The isolation and purification were carried out by many kinds of column chromatographic techniques. The flavonoids were analyzed by HPLC-DAD, and elucidated by 1H-NMR, 13C-NMR and ESI-MS.
Five compounds were obtained and therir structures were determined as kaempferol-3-O-glucoside (I), rutinoside (II), luteolin-7-O-glucoside (III), kaempferyl 5-methyl ether (IV), apigenin-5-O-neohesperidoside (V).
Compounds IV and V are isolated from this plant for the first time.
研究百蕊草有效部位中的黄酮类化合物的抗炎作用。
采用多种柱色谱技术进行分离纯化。通过高效液相色谱-二极管阵列检测器（HPLC-DAD）对黄酮类化合物进行分析，并用1H-NMR、13C-NMR和电喷雾电离质谱（ESI-MS）对其结构进行鉴定。
得到5个化合物，其结构分别鉴定为山柰酚-3-O-葡萄糖苷（I）、芸香苷（II）、木犀草素-7-O-葡萄糖苷（III）、山柰酚5-甲醚（IV）、芹菜素-5-O-新橙皮糖苷（V）。
化合物IV和V为首次从该植物中分离得到。
