Chang Hua-Lei, Lei Lin-Sheng, Yu Chuan-Lin, Zhu Zheng-Guang, Chen Na-Na, Wu Shu-Guang
Institute of Pharmaceutical Sciences, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China.
Zhong Yao Cai. 2009 Apr;32(4):561-3.
To study the effect of Flammulina velutipes polysaccharides (FVP) on the production of tumor necrosis factor alpha (TNF-alpha), interferon-gamma (INF-gamma) and interleukin 2 (IL-2) by murine immunocytes.
The cell's metabolic activity was determined with methylthiazolyl tetrazolium (MTT) colorimetry assay and the amounts of TNF-alpha, INF-gamma and IL-2 were measured by ELISA.
FVP (200, 100, 50 microg/mL) could promote the metabolic activity of murine splenocytes and peritoneal exudate cells (PEC) and increase the amounts of TNF-alpha, INF-gamma and IL-2 in the supernatants of splenocyte cultures, and the amount of TNF-alpha in PEC cultures, with the most marked increase on TNF-alpha level. FVP (100, 50, 25 mg/kg) could raise the serum levels of TNF-alpha and INF-gamma in S180 tumor-bearing mice.
FVP may regulate murine immune function through promoting the production of TNF-alpha, INF-gamma and IL-2.
研究金针菇多糖（FVP）对小鼠免疫细胞产生肿瘤坏死因子α（TNF-α）、干扰素-γ（INF-γ）和白细胞介素2（IL-2）的影响。
采用甲基噻唑基四氮唑（MTT）比色法测定细胞代谢活性，用酶联免疫吸附测定法（ELISA）检测TNF-α、INF-γ和IL-2的含量。
FVP（200、100、50微克/毫升）可促进小鼠脾细胞和腹腔渗出细胞（PEC）的代谢活性，增加脾细胞培养上清液中TNF-α、INF-γ和IL-2的含量，以及PEC培养物中TNF-α的含量，其中TNF-α水平升高最为显著。FVP（100、50、25毫克/千克）可提高S180荷瘤小鼠血清中TNF-α和INF-γ的水平。
FVP可能通过促进TNF-α、INF-γ和IL-2的产生来调节小鼠免疫功能。
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