[糙叶败酱提取物对肉瘤180腹水瘤荷瘤小鼠血清细胞因子水平的影响]

[Effect of Patrinia scabra extracts on the level of serum cytokine in sarcoma 180 ascitic tumor burdened mice].

作者信息

Chen Jian-Hua, Wang Xue-Xi

机构信息

Hospital of Pump Plant of Lanzhou, Lanzhou 730000, China.

出版信息

Zhong Yao Cai. 2008 Nov;31(11):1689-91.

PMID:19260282

Abstract

OBJECTIVE

To explore the effect of Potrinia scabro extracts (PSE) on the level of serum cytokine in Sarcoma 180 ascitic tumor burdened mice and its mechanism of anti-tumor.

METHODS

The mice model of Sarcoma 180 ascitic tumor were established and divided into five groups randomly, including the model group with normal saline solution, the positive group with 10 mg/kg cytoxan and PSE treated groups at doses of 2.0 g/kg, 1.0 g/kg, 0.5 g/kg intraperitoneally for 10 days. The level of serum cytokine Th1 (IL-2, IL-12, IFN-gamma, IFN-alpha) and Th2 (IL-6, IL-10) were measured by double antibody sandwich ELISA assay.

RESULTS

Compared with model group of Sarcoma 180 ascitic tumor burdened mice,the level of IL-2 and IFN-gamma increased in PSE 2.0 g/kg group, but the IL-6 and IL-10 decreased in PSE 2.0 g/kg and 1.0 mg/kg groups.

CONCLUSION

PSE has anti-tumor effect in vivo that could be related to the level variation of IL-2, IFN-gamma, IL-6 and IL-10 in tumor burdened mice.

摘要

目的

探讨糙叶紫堇提取物（PSE）对肉瘤180腹水瘤荷瘤小鼠血清细胞因子水平的影响及其抗肿瘤机制。

方法

建立肉瘤180腹水瘤小鼠模型，随机分为五组，包括生理盐水模型组、10 mg/kg环磷酰胺阳性组以及2.0 g/kg、1.0 g/kg、0.5 g/kg剂量PSE腹腔注射给药10天的处理组。采用双抗体夹心ELISA法检测血清细胞因子Th1（IL-2、IL-12、IFN-γ、IFN-α）和Th2（IL-6、IL-10）水平。