[微乳液法提取番石榴叶中槲皮素的研究]
[Study on extraction of quercetin in guava leaf by microemulsion].
作者信息
Lu Rui-Xin, Lu Wen-Biao
机构信息
Guangzhou University of Chinese Medicine, Guangzhou 510405, China.
出版信息
Zhong Yao Cai. 2009 Apr;32(4):608-10.
OBJECTIVE
To study the extraction of quercetin in guava leaf by microemulsion.
METHODS
The optimal component proportion was obtained by investigating the ratio of oil, water, surfactant and cosurfactant. The extraction of quercetin in microemulsion was determined by HPLC which compared with the traditional method.
RESULTS
The traditional solvent extraction methods were used to extract quercetin in guava leaf, and the content of quercetin extracted with methanol was the highest (4.72 microg/mL). But the contents of quercetin extracted by 72 percent of the microemulsions were higher than methanol, and the extraction by the optimum formulation of microemulsion which consisted of acetoacetate (oil phase) -30% avantin solution (water phase) -RH60 (surfactant) was 2 times as much as that was extracted by methanol (10.43 microg/mL).
CONCLUSION
Compared with traditional solvent extraction methods, microemulsion method can effectively improve the extraction rate of quercetin in guava leaf.
目的
研究微乳法提取番石榴叶中槲皮素的工艺。
方法
通过考察油相、水相、表面活性剂和助表面活性剂的比例，得出最佳组分比例。采用高效液相色谱法测定微乳中槲皮素的提取量，并与传统方法进行比较。
结果
采用传统溶剂提取法提取番石榴叶中的槲皮素，以甲醇提取的槲皮素含量最高（4.72μg/mL）。但72%的微乳提取的槲皮素含量高于甲醇，由乙酰乙酸乙酯（油相）-30%阿凡汀溶液（水相）-RH60（表面活性剂）组成的最佳微乳配方提取量是甲醇提取量的2倍（10.43μg/mL）。
结论
与传统溶剂提取法相比，微乳法能有效提高番石榴叶中槲皮素的提取率。
Zotero 插件