通过原子力显微镜测量的被囊动物单个纤维素微纤维的弹性模量。

Elastic modulus of single cellulose microfibrils from tunicate measured by atomic force microscopy.

作者信息

Iwamoto Shinichiro, Kai Weihua, Isogai Akira, Iwata Tadahisa

机构信息

Department of Biomaterial Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

出版信息

Biomacromolecules. 2009 Sep 14;10(9):2571-6. doi: 10.1021/bm900520n.

DOI:10.1021/bm900520n

PMID:19645441

Abstract

The elastic modulus of single microfibrils from tunicate ( Halocynthia papillosa ) cellulose was measured by atomic force microscopy (AFM). Microfibrils with cross-sectional dimensions 8 x 20 nm and several micrometers in length were obtained by oxidation of cellulose with 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO) as a catalyst and subsequent mechanical disintegration in water and by sulfuric acid hydrolysis. The nanocellulosic materials were deposited on a specially designed silicon wafer with grooves 227 nm in width, and a three-point bending test was applied to determine the elastic modulus using an AFM cantilever. The elastic moduli of single microfibrils prepared by TEMPO-oxidation and acid hydrolysis were 145.2 +/- 31.3 and 150.7 +/- 28.8 GPa, respectively. The result showed that the experimentally determined modulus of the highly crystalline tunicate microfibrils was in agreement with the elastic modulus of native cellulose crystals.

摘要

通过原子力显微镜（AFM）测量了来自被囊动物（Halocynthia papillosa）纤维素的单个微纤丝的弹性模量。通过以2,2,6,6-四甲基哌啶-1-氧基自由基（TEMPO）为催化剂氧化纤维素，随后在水中进行机械分解以及硫酸水解，获得了横截面尺寸为8×20 nm且长度为几微米的微纤丝。将纳米纤维素材料沉积在宽度为227 nm的特殊设计的硅片上，并使用AFM悬臂进行三点弯曲试验以确定弹性模量。通过TEMPO氧化和酸水解制备的单个微纤丝的弹性模量分别为145.2±31.3和150.7±28.8 GPa。结果表明，实验测定的高度结晶的被囊动物微纤丝的模量与天然纤维素晶体的弹性模量一致。