Yang Xin, Berry Tristan K, Foegeding E Allen
Dept. of Food, Bioprocessing and Nutrition Sciences, North Carolina State Univ., Raleigh, 27695-7624, USA.
J Food Sci. 2009 Jun;74(5):E259-68. doi: 10.1111/j.1750-3841.2009.01179.x.
Foams were prepared from whey protein isolate (WPI), egg white protein (EWP), and combinations of the 2 (WPI/EWP), with physical properties of foams (overrun, drainage 1/2 life, and yield stress), air/water interfaces (interfacial tension and interfacial dilatational elasticity), and foam microstructure (bubble size and dynamic change of bubble count per area) investigated. Foams made from EWP had higher yield stress and stability (drainage 1/2 life) than those made from WPI. Foams made from mixtures of EWP and WPI had intermediate values. Foam stability could be explained based on solution viscosity, interfacial characteristics, and initial bubble size. Likewise, foam yield stress was associated with interfacial dilatational elastic moduli, mean bubble diameter, and air phase fraction. Foams made from WPI or WPI/EWP combinations formed master curves for stability and yield stress when normalized according to the above-mentioned properties. However, EWP foams were excluded from the common trends observed for WPI and WPI/EWP combination foams. Changes in interfacial tension showed that even the lowest level of WPI substitution (25% WPI) was enough to cause the temporal pattern of interfacial tension to mimic the pattern of WPI instead of EWP, suggesting that whey proteins dominated the interface. The higher foam yield stress and drainage stability of EWP foams appears to be due to forming smaller, more stable bubbles, that are packed together into structures that are more resistant to deformation than those of WPI foams.
以乳清分离蛋白(WPI)、蛋清蛋白(EWP)以及二者的组合(WPI/EWP)制备泡沫,并对泡沫的物理性质(膨胀率、排水半衰期和屈服应力)、空气/水界面(界面张力和界面扩张弹性)以及泡沫微观结构(气泡大小和单位面积气泡数的动态变化)进行了研究。由EWP制成的泡沫比由WPI制成的泡沫具有更高的屈服应力和稳定性(排水半衰期)。由EWP和WPI混合物制成的泡沫具有中间值。泡沫稳定性可以基于溶液粘度、界面特性和初始气泡大小来解释。同样,泡沫屈服应力与界面扩张弹性模量、平均气泡直径和气相分数有关。当根据上述性质进行归一化时,由WPI或WPI/EWP组合制成的泡沫形成了稳定性和屈服应力的主曲线。然而,EWP泡沫被排除在WPI和WPI/EWP组合泡沫所观察到的共同趋势之外。界面张力的变化表明,即使是最低水平的WPI替代(25%WPI)也足以使界面张力的时间模式模仿WPI而不是EWP的模式,这表明乳清蛋白主导了界面。EWP泡沫较高的泡沫屈服应力和排水稳定性似乎是由于形成了更小、更稳定的气泡,这些气泡聚集在一起形成的结构比WPI泡沫的结构更抗变形。