State Key Laboratory for Biocontrol, Sun Yat-sen Zhongshan University, Guangzhou 510275, China.
Genomics. 2009 Nov;94(5):324-32. doi: 10.1016/j.ygeno.2009.07.006. Epub 2009 Jul 29.
Salt cress (Thellungiella halophila) has become a desirable plant model for molecular analysis of the mechanisms of salt tolerance. Analysis of its physiological action and expressed EST has resulted in better understanding. However, less is known about its genomic features. Here we determined a continuous sequence approximately 83 kb from a salt cress BAC clone, providing the first insight into the genomic feature for this species. The gene density is approximately one gene per 3.6 kb in this sequence. Many types of repetitive sequences are present in this salt cress sequence, including LTR retroelements, DNA transposons and a number of simple sequence repeats. Comparison of sequence similarity indicated that salt cress shares a close relationship with Arabidopsis. Extensive conservation and high-level microcolinearity were uncovered for both genomes. Our study also indicated that genomic DNA alternations (involving chromosome inversion, sequence loss and gene translocation) contributed to the genomic discrepancies between salt cress and Arabidopsis.
盐芥（Thellungiella halophila）已成为用于研究盐胁迫耐受机制的分子分析的理想植物模型。对其生理作用和表达 EST 的分析，加深了对其的了解。然而，关于其基因组特征的了解较少。在此，我们从盐芥 BAC 克隆中确定了一条约 83kb 的连续序列，为该物种的基因组特征提供了初步的认识。在该序列中，基因密度约为每 3.6kb 一个基因。该盐芥序列中存在多种类型的重复序列，包括 LTR 反转录元件、DNA 转座子和许多简单序列重复。序列相似性比较表明，盐芥与拟南芥关系密切。两个基因组之间存在广泛的保守性和高度的微共线性。我们的研究还表明，基因组 DNA 的改变（涉及染色体倒位、序列缺失和基因易位）导致了盐芥和拟南芥之间的基因组差异。
