Furness J B, Kuramoto H, Messenger J P
Department of Anatomy and Histology, Flinders University, Bedford Park, Australia.
J Auton Nerv Syst. 1990 Dec;31(3):203-10. doi: 10.1016/0165-1838(90)90186-m.
Labelled nerve cells were located in the distal colon of the guinea-pig 4-5 days after the retrograde tracing agent, Fast blue, was injected into the inferior mesenteric ganglia. Labelled neurons were only found in the myenteric plexus. Their frequency increased from oral to anal and was greater towards the mesenteric border, compared with the anti-mesenteric aspect, of the colon. Many retrogradely labelled neurons were immunoreactive for vasoactive intestinal peptide or calbindin. In the inferior mesenteric ganglia, vasoactive intestinal peptide and calbindin immunoreactive nerve fibres surrounded the same clumps of nerve cell bodies. Almost all calbindin and vasoactive intestinal peptide immunoreactive terminals degenerated after the nerves running from the large intestine to the inferior mesenteric ganglia were cut. It is concluded that the great majority of calbindin and vasoactive intestinal peptide immunoreactive terminals in the inferior mesenteric ganglia arise from nerve cell bodies in the myenteric plexus of the large intestine.
在将逆行示踪剂快蓝注入肠系膜下神经节后4 - 5天，在豚鼠的远端结肠中发现了标记的神经细胞。标记的神经元仅在肌间神经丛中被发现。它们的频率从口侧到肛侧增加，并且与结肠的系膜对侧相比，在靠近肠系膜边界处更多。许多逆行标记的神经元对血管活性肠肽或钙结合蛋白具有免疫反应性。在肠系膜下神经节中，血管活性肠肽和钙结合蛋白免疫反应性神经纤维围绕着相同的神经细胞体团块。在切断从大肠到肠系膜下神经节的神经后，几乎所有钙结合蛋白和血管活性肠肽免疫反应性终末都发生了退变。结论是，肠系膜下神经节中绝大多数钙结合蛋白和血管活性肠肽免疫反应性终末起源于大肠肌间神经丛中的神经细胞体。
