Nishino Maki, Mizuno Dai, Kimoto Takashi, Shinahara Wakako, Fukuta Akiho, Takei Tsunetomo, Sumida Kaori, Kitamura Seiichiro, Shiota Hiroshi, Kido Hiroshi
Division of Enzyme Chemistry, Institute for Enzyme Research, The University of Tokushima, Kuramoto-cho 3-18-15, Tokushima 770-8503, Japan.
Vaccine. 2009 Sep 18;27(41):5620-7. doi: 10.1016/j.vaccine.2009.07.024. Epub 2009 Jul 30.
Immune responses and side effects of intranasally administered flu vaccine with the commercial product Surfacten, a modified bovine pulmonary surfactant, were investigated in minipigs. The use of minipigs was based on the anatomical resemblance of nasal lymph nodes, the principal antigen uptake site of respiratory mucosal immunity, between pig and human. Intranasal instillation of HA vaccine adjuvanted with Surfacten elicited significantly higher serum hemagglutination inhibition titers than the antigen alone, with wide cross-neutralizing activities of secretory IgA in nasal washes. No significant induction of inflammatory cytokines or migration of inflammatory cells was observed at the site of immunization or serum after the first immunization. These data suggest the potential usefulness of Surfacten for mucosal vaccination.
在小型猪中研究了经鼻给予流感疫苗与商业产品Surfacten（一种改良的牛肺表面活性剂）的免疫反应和副作用。使用小型猪是基于猪与人之间鼻淋巴结（呼吸道黏膜免疫的主要抗原摄取部位）在解剖学上的相似性。经鼻滴注用Surfacten佐剂的HA疫苗比单独的抗原引发了显著更高的血清血凝抑制效价，在鼻腔灌洗液中分泌型IgA具有广泛的交叉中和活性。首次免疫后，在免疫部位或血清中未观察到炎性细胞因子的显著诱导或炎性细胞的迁移。这些数据表明Surfacten在黏膜疫苗接种方面具有潜在的应用价值。
