Neves Patrícia Cristina da Costa, Matos Denise Cristina de Souza, Marcovistz Rugimar, Galler Ricardo
Vice-diretoria de Desenvolvimento Tecnológico, Bio-Manguinhos, Fundação Oswaldo Cruz, Manguinhos 21040-360, Rio de Janeiro, Brazil.
Vaccine. 2009 Sep 18;27(41):5543-9. doi: 10.1016/j.vaccine.2009.07.028. Epub 2009 Jul 30.
The yellow fever vaccine is very effective with a single injection conferring protection for at least 10 years. Recent evidence suggests that the innate immune cells activated through Toll-like receptors (TLRs), are critical determinants of the robustness of the adaptive response. Therefore, we investigated the NK cell status in eight healthy volunteers after vaccination with YF 17DD virus. Shortly after vaccination, we observed increased expression of TLR-3 and TLR-9 in NK cells and markers such as CD69, HLA-DP-DQ-DR, CD38 and CD16. The up-regulation of CD69 was positively correlated with the presence of TLRs throughout the post-vaccination period and the circulating IFN-gamma was significantly augmented. These results suggest that TLRs may play an important role in NK cell activation during the immune response to vaccination, indicating a potential role for NK cells in helping the development of long-lasting protective memory.
黄热病疫苗单次注射非常有效，可提供至少10年的保护。最近的证据表明，通过Toll样受体（TLR）激活的先天免疫细胞是适应性反应强度的关键决定因素。因此，我们研究了8名健康志愿者接种YF 17DD病毒疫苗后的自然杀伤（NK）细胞状态。接种疫苗后不久，我们观察到NK细胞中TLR-3和TLR-9以及CD69、HLA-DP-DQ-DR、CD38和CD16等标志物的表达增加。在整个疫苗接种后期间，CD69的上调与TLR的存在呈正相关，并且循环中的γ干扰素显著增加。这些结果表明，TLR可能在疫苗接种免疫反应期间的NK细胞激活中发挥重要作用，这表明NK细胞在帮助形成持久保护性记忆方面具有潜在作用。
