Albano R M, Pavão M S, Mourão P A, Mulloy B
Departamento de Bioquimica, Universidade Federal do Rio de Janeiro, Brazil.
Carbohydr Res. 1990 Dec 15;208:163-74. doi: 10.1016/0008-6215(90)80096-l.
The tunic of the ascidian Styela plicata is rich in a high molecular weight sulfated-L-galactan called the F-1 fraction. This polysaccharide is of complex structure and is highly branched. In this study we undertook detailed structural analysis of the F-1 fraction that was submitted to the Smith degradation procedure which, for this polysaccharide, results mainly in the elimination of the branches. By methylation, and one- and two-dimensional n.m.r. analysis of the Smith-degraded and desulfated Smith-degraded F-1 fraction, we determined the main structure of this polymer. It is composed of a core of alpha-L-galactopyranose units, sulfated at C-3 and glycosidically linked through position 1----4. The nonsulfated, nonreducing end units are branched at C-2 of the sulfated L-galactoses.
皱瘤海鞘的被囊富含一种称为F-1组分的高分子量硫酸化L-半乳聚糖。这种多糖结构复杂且高度分支。在本研究中，我们对F-1组分进行了详细的结构分析，该组分经过史密斯降解程序，对于这种多糖而言，该程序主要导致分支的消除。通过对史密斯降解和脱硫酸史密斯降解的F-1组分进行甲基化以及一维和二维核磁共振分析，我们确定了这种聚合物的主要结构。它由α-L-吡喃半乳糖单元的核心组成，在C-3位硫酸化并通过1→4位糖苷键连接。非硫酸化、非还原端单元在硫酸化L-半乳糖的C-2位分支。
