[肾盂肾炎患儿尿液中的基质金属蛋白酶2和9及其组织抑制剂1和2]
[Matrix metalloproteinases 2 and 9 and their tissue inhibitors 1 and 2 in the urine of children with pyelonephritis].
作者信息
Tenderenda Edyta, Zoch-Zwierz Walentyna, Wasilewska Anna, Porowski Tadeusz, Taranta-Janusz Katarzyna, Kołodziejczyk Zbigniew, Michaluk-Skutnik Joanna
机构信息
Uniwersytet Medyczny w Białymstoku, Klinika Pediatrii i Nefrologii.
出版信息
Pol Merkur Lekarski. 2009 Jul;27(157):10-3.
UNLABELLED
In small children, pyelonephritis (PN) is an important cause of scarring in the renal and disturbed in the production and degradation of extracellulare matrix proteins (ECM). Aim of the study was to assess the urinary levels metalloproteinases 2 and 9 (MMP-2 and MMP-9) and their inhibitors 1 and 2 (TIMP-1 and TIMP-2) in children with pyelonephritis (PN).
MATERIALS AND METHODS
Study group (I) consisted of 42 children with PN, aged 1-15 years, examined twice: A--prior to treatment (1-3 days of fever), B--after antibacterial treatment (10-14 days). The control group (K) consisted of 30 healthy children. Enzyme-linked immunosorbent assay kits were used for measurements of total human MMP-2, MMP-9, TIMP-1 and TIMP-2 in first morning urine.
RESULTS
In children with PN (I) prior to treatment (A), urinary concentration of all parameters were increased as compared to the control (K) (p<0.05). After treatment (B), only the levels of TIMP-1 was still elevated (p = 0.02). In PN before (A) and after (B) treatment MMP-9/TIMP-1 ratio. However MMP-2/TIMP-2 ratio was normal.
CONCLUSION
In children with PN the balance MMP-9/TIMP-1 is disturbed, with the predominance of TIMP-1 production over MMP-9. It may lead to renal fibrosis.
未标注
在幼儿中，肾盂肾炎（PN）是导致肾脏瘢痕形成的重要原因，且细胞外基质蛋白（ECM）的产生和降解也会受到干扰。本研究的目的是评估肾盂肾炎（PN）患儿尿液中金属蛋白酶2和9（MMP - 2和MMP - 9）及其抑制剂1和2（TIMP - 1和TIMP - 2）的水平。
材料与方法
研究组（I）由42例年龄在1至15岁的PN患儿组成，进行了两次检查：A——治疗前（发热1 - 3天），B——抗菌治疗后（10 - 14天）。对照组（K）由30名健康儿童组成。使用酶联免疫吸附测定试剂盒检测晨尿中总人MMP - 2、MMP - 9、TIMP - 1和TIMP - 2的含量。
结果
PN患儿（I）治疗前（A），所有参数的尿浓度与对照组（K）相比均升高（p<0.05）。治疗后（B），仅TIMP - 1水平仍升高（p = 0.02）。在PN治疗前（A）和治疗后（B），MMP - 9/TIMP - 1比值存在差异。然而，MMP - 2/TIMP - 2比值正常。
结论
PN患儿中MMP - 9/TIMP - 1平衡受到干扰，TIMP - 1的产生超过MMP - 9。这可能导致肾纤维化。
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