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[利用傅里叶变换红外光谱法对经太空诱变的第四代黄芩进行分析]

[Analysis of the 4th generation Scutellaria baicalensis Georgi with space mutagenesis via FTIR spectroscopy].

作者信息

Ding Xi-feng, Gao Hua-na, Guo Xi-hua, Wang Zhi-zhou, Yang La-huz, Guan Ying, Shi Jin-shan

机构信息

College of Sciences, Yanshan University, Qinhuangdao 066004, China.

出版信息

Guang Pu Xue Yu Guang Pu Fen Xi. 2009 May;29(5):1286-8.

Abstract

Determination and analysis for the Scutellaria baicalensis Georgi with space mutagenesis and the ground group were carried out with FTIR for the first time in order to fully understand the quality changes of the 4th generation of space mutagenesis of Scutellaria baicalensis Georgi and do further research. The result shows that for the FTIR spectra of the two samples the position and shape of main absorption peaks are approximately the same, indicating that the major components and the structures of space group remain intact, but the intensities of the absorption peaks are obviously different, with the intensities of the space group significantly enhanced compared to the ground group, especially for the flavonoid compounds (baicalin, baicalein, wogonoside, wogonin and wogonoside etc), which are the main active components of the Scutellaria baicalensis Georgi, the absorption peak intensities at 3391, 1655 and 1069 cm(-1) are obviously stronger than those of the ground group, so the contents of flavonoid compounds and other components are higher, and the amorphous active components are optimized. Space mutation breeding is conducive to breeding new varieties of highly active components, and it is also one of the ways to innovate Chinese medicines germplasm resources efficiently.

摘要

首次采用傅里叶变换红外光谱法(FTIR)对太空诱变黄芩及其地面对照组进行测定与分析,以全面了解黄芩太空诱变第4代的品质变化并做进一步研究。结果表明,两个样品的FTIR光谱中,主要吸收峰的位置和形状大致相同,表明太空组的主要成分和结构保持完整,但吸收峰的强度明显不同,太空组的强度相较于地面对照组显著增强,尤其是对于黄酮类化合物(黄芩苷、黄芩素、汉黄芩苷、汉黄芩素和千层纸苷等),它们是黄芩的主要活性成分,在3391、1655和1069 cm(-1)处的吸收峰强度明显强于地面对照组,因此黄酮类化合物及其他成分的含量更高,无定形活性成分得到优化。太空诱变育种有利于培育高活性成分的新品种,也是高效创新中药材种质资源的途径之一。

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