Chen Weiwei, Yu Man, Wang Yun, Peng Youwei, Li Xiaokun, Lam Dominic Man-Kit, Chen Xiaoming, Liu Xuyang
Department of Ophthalmology and Ophthalmic Laboratories, West China Hospital, Sichuan University, Chengdu, People's Republic of China.
J Ocul Pharmacol Ther. 2009 Aug;25(4):315-20. doi: 10.1089/jop.2009.0015.
To investigate the protective effects of non-mitogenic human acidic fibroblast growth factor on retinal degeneration induced by NaIO(3) in rats.
Retinal degeneration was induced in adult male Wistar rats via caudal-vein injection of 1% NaIO(3) at 50 mg/kg. One h after NaIO(3) treatment, the right eyes received intravitreal injection of 2.5 microg nm-haFGF in 10 microL saline and the left eyes received saline alone as vehicle-treated eyes. Retinal function in rats was evaluated by electroretinogram (ERG) before injection and 1, 7, and 21 days postinjection. Additional rat eyes were enucleated 7 and 21 days postinjection, fixed, and processed for histological examination.
A model of retinal degeneration in rat was established successfully using NaIO(3) injection. Significant decreases in both ERG a- and b-wave amplitudes were detected in NaIO(3)-injected rats when compared with the normal animals (P < 0.05) on day 7 postinjection. Importantly, at the seventh day after intravitreal nm-haFGF treatment on NaIO(3)-injected rats, the nm-haFGF-treated eyes showed a significant improvement in the ERG amplitudes of both a- and b-waves when compared with vehicle-treated eyes (P < 0.05). In addition, the disruptions of photoreceptor outer segments and the retinal pigment epithelium monolayer were much less frequently observed in the nm-haFGF-treated eyes than the vehicle-treated eyes, and the outer nuclear layer thickness in the nm-haFGF-treated eyes was similar to that of the normal eyes.
Intravitreal delivery of nm-haFGF appears to have neuroprotective effect on retinal degeneration induced by NaIO(3).
研究非促有丝分裂的人酸性成纤维细胞生长因子对大鼠碘酸钠诱导的视网膜变性的保护作用。
通过尾静脉注射50mg/kg的1%碘酸钠诱导成年雄性Wistar大鼠发生视网膜变性。碘酸钠治疗1小时后，右眼玻璃体腔内注射10μL盐水中含2.5μg非促有丝分裂人酸性成纤维细胞生长因子（nm-haFGF），左眼仅注射盐水作为对照。在注射前以及注射后1天、7天和21天通过视网膜电图（ERG）评估大鼠的视网膜功能。在注射后7天和21天摘除额外的大鼠眼球，固定并进行组织学检查。
通过注射碘酸钠成功建立了大鼠视网膜变性模型。与正常动物相比，注射碘酸钠的大鼠在注射后第7天ERG a波和b波振幅均显著降低（P<0.05）。重要的是，在注射碘酸钠的大鼠玻璃体腔内注射nm-haFGF治疗7天后，与对照眼相比，nm-haFGF治疗眼的ERG a波和b波振幅均有显著改善（P<0.05）。此外，与对照眼相比，nm-haFGF治疗眼较少观察到光感受器外段和视网膜色素上皮单层的破坏，nm-haFGF治疗眼的外核层厚度与正常眼相似。
玻璃体腔内注射nm-haFGF对碘酸钠诱导的视网膜变性似乎具有神经保护作用。
