Department of Internal Medicine, Endocrinology and Metabolism Unit, Hacettepe University School of Medicine, Hacettepe, Ankara, Turkey.
Clin Endocrinol (Oxf). 2010 Apr;72(4):469-74. doi: 10.1111/j.1365-2265.2009.03674.x. Epub 2009 Jul 24.
High molecular weight adiponectin (HMWA) is the active circulating form of adiponectin. Nampt/visfatin is the enzyme secreted from adipocytes in an active form and is one of the putative regulators of insulin secretion.
To investigate the dynamics of total adiponectin (TA), HMWA and Nampt/visfatin in obese and lean women during oral glucose tolerance test (OGTT).
We studied normal glucose-tolerant (NGT), age-matched, 30 obese and 30 lean women. All subjects underwent a standard 75 g, 2-h OGTT, and area under the curve (AUC) during OGTT for glucose, insulin, Nampt/visfatin, TA and HMWA was calculated. Body fat mass was assessed by bioimpedance analysis. Results Obese women had significantly higher basal and AUC values for insulin and Nampt/visfatin, whereas basal and AUC-HMWA were significantly lower in this group. Alternatively, obese and lean groups had similar basal and AUC values for glucose and TA. Basal insulin levels were negatively correlated with HMWA levels, but not with basal Nampt/visfatin. AUC-insulin was correlated positively with AUC-visfatin, and negatively with AUC-HMWA. Total and truncal body fat mass showed positive correlation with basal and AUC-visfatin, and negative correlation with basal and AUC-HMWA.
In the NGT state, obese women have higher Nampt/visfatin and lower HMWA levels, both basally and in response to oral glucose challenge. The dynamics of Nampt/visfatin and HMWA during OGTT appear to be linked with insulin and adiposity. Counter-regulatory adaptations in HMWA and Nampt/visfatin might have an impact on suggested adipoinsular axis, contributing to maintenance of normal glucose tolerance.
高分子量脂联素（HMWA）是脂联素的活性循环形式。Nampt/visfatin 是脂肪细胞分泌的一种活性形式的酶，是胰岛素分泌的潜在调节因子之一。
研究肥胖和瘦女性在口服葡萄糖耐量试验（OGTT）过程中总脂联素（TA）、HMWA 和 Nampt/visfatin 的动态变化。
我们研究了正常糖耐量（NGT）、年龄匹配的 30 名肥胖女性和 30 名瘦女性。所有受试者均进行了标准的 75g、2hOGTT，并计算了 OGTT 期间葡萄糖、胰岛素、Nampt/visfatin、TA 和 HMWA 的曲线下面积（AUC）。体脂量通过生物阻抗分析进行评估。结果肥胖女性的基础和 AUC 值胰岛素和 Nampt/visfatin 显著升高，而基础和 AUC-HMWA 则显著降低。相反，肥胖组和瘦组的基础和 AUC 值葡萄糖和 TA 相似。基础胰岛素水平与 HMWA 水平呈负相关，但与基础 Nampt/visfatin 水平无关。AUC-胰岛素与 AUC-visfatin 呈正相关，与 AUC-HMWA 呈负相关。总脂肪量和躯干脂肪量与基础和 AUC-visfatin 呈正相关，与基础和 AUC-HMWA 呈负相关。
在 NGT 状态下，肥胖女性的基础和口服葡萄糖刺激后，Nampt/visfatin 水平升高，HMWA 水平降低。OGTT 期间 Nampt/visfatin 和 HMWA 的动态变化似乎与胰岛素和肥胖有关。HMWA 和 Nampt/visfatin 的代偿性适应可能对拟议的脂肪胰岛轴产生影响，有助于维持正常糖耐量。
