Li Jia, Zhang Bing, Zhong Li-Li
Department of Pediatrics, Hunan Provincial People's Hospital, Changsha 410005, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2009 Jul;11(7):577-80.
To study the expression of human beta-defensin-3 (hBD-3) induced by lipopolysaccharide (LPS) in human bronchial epithelial (HBE) cells, and explore the role of hBD-3 in respiratory infection.
HBE cells were stimulated with different concentrations of LPS (0.01, 0.1, 1 and 10 microg/mL). hBD-3 mRNA expression was detected by RT-PCR 2 hrs later. hBD-3 protein expression was detected by Western blot 4 hrs later.
hBD-3 mRNA and protein was weakly expressed in normal HBE cells. LPS stimulation resulted in a significant increase of hBD-3 mRNA and protein expression (p<0.01). hBD-3 mRNA and protein expression increased with increasing LPS concentrations. There were significant differences in the hBD-3 mRNA and protein expression in cells stimulated by different concentrations of LPS (p<0.05).
LPS can induce hBD-3 expression in a dose-dependent manner. hBD-3 might play a role in initial defensive reaction against bacterial invasion.
研究脂多糖（LPS）诱导人支气管上皮（HBE）细胞中人类β-防御素-3（hBD-3）的表达情况，并探讨hBD-3在呼吸道感染中的作用。
用不同浓度的LPS（0.01、0.1、1和10微克/毫升）刺激HBE细胞。2小时后通过逆转录聚合酶链反应（RT-PCR）检测hBD-3 mRNA的表达。4小时后通过蛋白质免疫印迹法检测hBD-3蛋白的表达。
正常HBE细胞中hBD-3 mRNA和蛋白呈弱表达。LPS刺激导致hBD-3 mRNA和蛋白表达显著增加（p<0.01）。hBD-3 mRNA和蛋白表达随LPS浓度增加而增加。不同浓度LPS刺激的细胞中hBD-3 mRNA和蛋白表达存在显著差异（p<0.05）。
LPS能以剂量依赖方式诱导hBD-3表达。hBD-3可能在针对细菌入侵的初始防御反应中发挥作用。
