Barbosa Helder, Slater Nigel K H, Marcos João C
Center of Chemistry, University of Minho, Braga, Portugal.
Anal Biochem. 2009 Dec 1;395(1):108-10. doi: 10.1016/j.ab.2009.07.045. Epub 2009 Aug 3.
Some experimental methodologies require the quantification of protein in the presence of polymers like poly(ethylene glycol) (PEG) and dextran (DEX). In the aqueous two-phase system (ATPS) extraction of biomolecules, the interference of these phase-forming polymers on the Bradford quantification assay is commonly recognized. However, how these polymers interfere has not been reported hitherto. In this study we show that while dextran concentrations of 20% (w/w) can be used without error, loss of accuracy occurs for solutions with PEG concentrations >10% (w/w). Above this value a substantial decrease on the assay sensitivity is observed.
一些实验方法需要在存在聚乙二醇（PEG）和葡聚糖（DEX）等聚合物的情况下对蛋白质进行定量。在生物分子的双水相系统（ATPS）萃取中，这些成相聚合物对考马斯亮蓝定量测定的干扰是普遍公认的。然而，迄今为止尚未报道这些聚合物是如何产生干扰的。在本研究中，我们表明虽然20%（w/w）的葡聚糖浓度可以无误地使用，但对于PEG浓度>10%（w/w）的溶液会出现准确性损失。高于此值时，可观察到测定灵敏度大幅下降。
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