Pathology Department, "ATTIKON" University Hospital, University of Athens Medical School, 1 Rimini St, 12462, Athens, Greece.
Int Urol Nephrol. 2010 Jun;42(2):325-30. doi: 10.1007/s11255-009-9622-1. Epub 2009 Aug 5.
We aimed to determine whether a standard P504S, 34betaE12 and p63 immunostaining of prostate core needle biopsy specimens can optimize diagnostic accuracy of conventional staining methods.
The staining properties of all three antibodies were evaluated on 250 prostate biopsies formerly diagnosed as benign.
Lack of basal cell staining in more than three glands for 34betaE12 and p63 occurred in 41 (27.5%) and 9 (6%) respective cases from the General Hospital pool. Respective figures from the Uropathology department specimens were 18 (18%) for 34betaE12 and 8 (8%) for p63. With the aid of P504S positivity, a case of prostate cancer as well as another of atypical small acinar proliferation was discovered.
Despite its potential for important aid in accurate diagnosis, standard application of immunohistochemistry in prostate biopsy is not justified and should be reserved for equivocal cases where conventional pathology fails to be conclusive.
我们旨在确定前列腺核心针活检标本的标准 P504S、34βE12 和 p63 免疫染色是否可以优化常规染色方法的诊断准确性。
在 250 份以前诊断为良性的前列腺活检标本上评估了所有三种抗体的染色特性。
在综合医院组的 41 例（27.5%）和 9 例（6%）分别出现了 34βE12 和 p63 超过三个腺体缺乏基底细胞染色的情况。泌尿科标本的相应数字分别为 34βE12 的 18 例（18%）和 p63 的 8 例（8%）。借助 P504S 阳性，发现了一例前列腺癌和另一例非典型小腺泡增生。
尽管免疫组织化学在前列腺活检中的标准应用具有重要的辅助诊断作用，但仍没有得到证实，不应常规应用，而应保留用于常规病理学无法得出明确结论的可疑病例。
