Ko Huey-Jiun, Song Airong, Lai Min-Nan, Ng Lean-Teik
Department of Food Science, National Chiayi University, Chiayi, Taiwan.
Am J Chin Med. 2009;37(4):815-28. doi: 10.1142/S0192415X09007260.
The present study aimed to investigate the antioxidant and antiradical activities of Wu Ling Shen, a popular medicinal fungus (Xylaria nigripes) used in traditional Chinese medicine preparations. Two different X. nigripes materials, the cultivated X. nigripes mycelia (XN) and a commercial X. nigripes product (XNP), were used to prepare the aqueous (XN-H vs. XNP-H) and ethanol (XN-E vs. XNP-E) extracts for this study. Polyphenol and total polysaccharide contents of these extracts were also examined. Results showed that extracts of XN possessed stronger antioxidant and antiradical activities than XNP in all tested model systems. However, all extracts exhibited a weak activity in metal chelation and reducing power. Total antioxidant activity of XN extracts (IC50 6.20 microg/ml for XN-H and 5.41 microg/ml for XN-E), but not XNP extracts (IC50 128.13 microg/ml for XNP-H and 96.16 microg/ml for XNP-E), was more potent than Trolox (IC50 19.64 microg/ml) and vitamin C (IC50 26.39 microg/ml). XN-E (IC50 5.12 microg/ml) and XNP-E (IC50 8.89 microg/ml) possessed a relatively similar potency as that of positive controls (IC50 6.94 microg/ml for Trolox and 4.25 microg/ml for vitamin C) in the superoxide radical scavenging activity. Although the DPPH radical scavenging of XN extracts was weaker than that of Trolox and vitamin C, it was about eight times more potent than that of XNP extracts. In ABTS assay, both XN and XNP extracts exhibited a moderate ABTS radical scavenging activity. Among the different extracts, XN-E showed the highest total flavonoid (32.69 mg/g) and phenol (59.75 mg/g) contents, while XNP-H (7.50% w/w) had the highest level in total polysaccharide content. These results conclude that XN-E possesses the most potent antioxidant and antiradical activities, and that these activities could be derived from its high polyphenol content, but not the level of polysaccharides.
本研究旨在探究乌灵参（一种用于中药制剂的常见药用真菌黑柄炭角菌）的抗氧化和抗自由基活性。本研究使用了两种不同的黑柄炭角菌材料，即人工栽培的黑柄炭角菌菌丝体（XN）和一种市售黑柄炭角菌产品（XNP），来制备水提取物（XN-H与XNP-H）和乙醇提取物（XN-E与XNP-E）。还检测了这些提取物中的多酚和总多糖含量。结果表明，在所有测试模型系统中，XN提取物的抗氧化和抗自由基活性均强于XNP提取物。然而，所有提取物在金属螯合和还原能力方面均表现出较弱的活性。XN提取物的总抗氧化活性（XN-H的IC50为6.20微克/毫升，XN-E的IC50为5.41微克/毫升），而不是XNP提取物（XNP-H的IC50为128.13微克/毫升，XNP-E的IC50为96.16微克/毫升），比Trolox（IC50为19.64微克/毫升）和维生素C（IC50为26.39微克/毫升）更强效。XN-E（IC50为5.12微克/毫升）和XNP-E（IC50为8.89微克/毫升）在超氧阴离子自由基清除活性方面与阳性对照（Trolox的IC50为6.94微克/毫升，维生素C的IC50为4.25微克/毫升）具有相对相似的效力。虽然XN提取物对DPPH自由基的清除能力弱于Trolox和维生素C，但其效力约为XNP提取物的八倍。在ABTS测定中，XN和XNP提取物均表现出中等程度的ABTS自由基清除活性。在不同提取物中，XN-E的总黄酮（32.69毫克/克）和酚类（59.75毫克/克）含量最高，而XNP-H的总多糖含量最高（7.50% w/w）。这些结果表明，XN-E具有最强的抗氧化和抗自由基活性，且这些活性可能源自其高多酚含量，而非多糖水平。
