Zhao Zhengqing, Li Yanpeng, Chen Haiyan, Huang Liuqing, Zhao Fei, Yu Qiang, Xiang Zhenghua, Zhao Zhongxin
Department of Neurology, Institute of Neuroscience and MOE Key Laboratory of Molecular Neurobiology, Neuroscience Research Center of Changzheng Hospital, Second Military Medical University Shanghai, PR China.
Department of Endocrinology, Changzheng Hospital, Second Military Medical University Shanghai, PR China.
Int J Clin Exp Med. 2014 Feb 15;7(2):356-62. eCollection 2014.
We aimed to investigate the effects of Xylaria nigripes (XN) extracts on the rapid eye movement sleep deprivation (REMSD)-induced memory impairment, and explore related mechanism. Male Sprague Dawley rats were randomly divided into 6 groups: cage control (CC)-NaCl group; tank control (TC)-NaCl group; sleep deprivation (SD)-NaCl group; CC-XN group; TC-XN group and SD-XN group. The rats were administered with intragastric XN and 0.9% of sodium chloride. SD group rats were deprived of REM sleep for 72 h. Morris water maze (MWM) was used to assess the effects of XN on spatial learning and memory. The expression of cAMP-response element binding protein (CREB) and p-CREB were also investigated in all groups. Result showed rats in SD-NaCl group had significantly longer mean escape latencies in finding the platform as compared to the control rats (p<0.05) in MWM test. The SD-NaCl group spent significantly less time in goal quadrant compared with the SD-XN group. REMSD and XN did not alter CREB expression in the hippocampus, while sleep deprivation resulted in reduced phosphorylation of CREB in the hippocampus, which was reversed by XN. XN mitigates spatial memory impairment induced by REMSD in rat. Phosphorylation of CREB in hippocampus might be one of the mechanisms.
我们旨在研究黑柄炭角菌（XN）提取物对快速眼动睡眠剥夺（REMSD）诱导的记忆损伤的影响，并探索相关机制。雄性Sprague Dawley大鼠被随机分为6组：笼养对照（CC）-NaCl组；水槽对照（TC）-NaCl组；睡眠剥夺（SD）-NaCl组；CC-XN组；TC-XN组和SD-XN组。给大鼠灌胃XN和0.9%的氯化钠。SD组大鼠被剥夺快速眼动睡眠72小时。采用莫里斯水迷宫（MWM）评估XN对空间学习和记忆的影响。还检测了所有组中环磷酸腺苷反应元件结合蛋白（CREB）和磷酸化CREB（p-CREB）的表达。结果显示，在MWM试验中，与对照大鼠相比，SD-NaCl组大鼠找到平台的平均逃避潜伏期显著延长（p<0.05）。与SD-XN组相比，SD-NaCl组在目标象限花费的时间显著减少。REMSD和XN均未改变海马中CREB的表达，而睡眠剥夺导致海马中CREB磷酸化减少，XN可使其逆转。XN减轻了大鼠中由REMSD诱导的空间记忆损伤。海马中CREB的磷酸化可能是其机制之一。
