Marley M S D, Givens M D, Galik P K, Riddell K P, Stringfellow D A
Department of Pathobiology, Animal Health Research, College of Veterinary Medicine, Auburn University, AL 36849, USA.
Reprod Domest Anim. 2009 Jun;44(3):532-5. doi: 10.1111/j.1439-0531.2008.01108.x.
The purpose of this study was to determine whether or not embryos derived from in vitro fertilization of oocytes from persistently infected (PI) cattle would contain infectious virus.Three in vitro embryo production treatment groups were assessed: 1) oocytes and uterine tubal cells (UTC) free of bovine viral diarrhoea virus (BVDV) (negative control), 2)oocytes free of BVDV fertilized and cultured in media containing UTC obtained from PI heifers, and 3) oocytes from PI heifers fertilized and cultured in media containing UTC free of BVDV. The developmental media, UTC and embryos (individual or groups of five) were assayed for virus.Virus was not isolated from any samples in treatment group 1.As shown in previous studies, a proportion of embryo samples were positive for BVDV in treatment group 2. In treatment group 3, the virus associated with the oocytes contaminated the developmental media and infected susceptible co-culture cells used during fertilization and culture. In addition, 65% (11/17) of the degenerated ova from treatment group 3 had infectious virus associated with them. While none of the ova developed into transferable embryos, the study did confirm that use of oocytes from PI cows could lead to amplification of BVDV and cross contamination during in vitro embryo production.
本研究的目的是确定来自持续感染（PI）牛的卵母细胞体外受精产生的胚胎是否会含有传染性病毒。评估了三个体外胚胎生产治疗组：1）不含牛病毒性腹泻病毒（BVDV）的卵母细胞和输卵管细胞（UTC）（阴性对照），2）在含有从PI小母牛获得的UTC的培养基中受精和培养的不含BVDV的卵母细胞，以及3）在含有不含BVDV的UTC的培养基中受精和培养的PI小母牛的卵母细胞。对发育培养基、UTC和胚胎（单个或五个一组）进行病毒检测。在治疗组1的任何样品中均未分离出病毒。如先前研究所示，治疗组2中的一部分胚胎样品BVDV呈阳性。在治疗组3中，与卵母细胞相关的病毒污染了发育培养基，并感染了受精和培养过程中使用的易感共培养细胞。此外，治疗组3中65%（11/17）的退化卵母细胞携带传染性病毒。虽然没有一个卵母细胞发育成可移植胚胎，但该研究确实证实，使用PI母牛的卵母细胞可能导致BVDV的扩增和体外胚胎生产过程中的交叉污染。
