Departamento de Quimica Analitica, Facultad de Quimica, Universidad de Murcia, Murcia, Spain.
J Agric Food Chem. 2009 Aug 26;57(16):7245-9. doi: 10.1021/jf901726e.
A sensitive, selective and solvent-free procedure is proposed for the rapid determination of monophosphate nucleotides (cytidine 5'-monophosphate, uridine 5'-monophosphate, adenosine 5'-monophosphate, and guanosine 5'-monophosphate) in baby foods. The method is based on the deproteinization of foods and direct analysis by anion exchange liquid chromatography (LC). Nucleotides were separated on an SAX anion exchange column with isocratic elution using 0.01 M dihydrogenphosphate buffer (pH 3.5) as mobile phase at a flow-rate of 1 mL min(-1) and detected by diode-array detection (DAD). The LC method rapidly separated the nucleotides (9 min) and was validated for linearity, detection and quantitation limits, selectivity, accuracy, and precision. The recoveries obtained for spiked samples were satisfactory for all the analytes. The proposed procedure allowed the only authorized nucleotides in infant formulas to be determined and was successfully applied to the analysis of different baby and/or functional food samples, including infant formulas, fermented milk, cereals, and purees.
本文提出了一种灵敏、选择性好且无需溶剂的方法，用于快速测定婴儿食品中的单磷酸核苷酸（胞苷 5'-单磷酸、尿苷 5'-单磷酸、腺苷 5'-单磷酸和鸟苷 5'-单磷酸）。该方法基于食品的除蛋白作用和直接用阴离子交换液相色谱（LC）分析。核苷酸在 SAX 阴离子交换柱上进行分离，采用等度洗脱，以 0.01 M 磷酸二氢盐缓冲液（pH 3.5）作为流动相，流速为 1 mL min(-1)，并用二极管阵列检测（DAD）进行检测。LC 方法能快速分离核苷酸（9 min），并对线性、检测限和定量限、选择性、准确度和精密度进行了验证。添加标准样品的回收率对所有分析物均令人满意。该方法仅允许在婴儿配方食品中添加已授权的核苷酸，并成功应用于不同婴儿和/或功能性食品样品的分析，包括婴儿配方食品、发酵乳、谷物和泥状食品。
