Panagiotopoulos I A, Bakker R R, Budde M A W, de Vrije T, Claassen P A M, Koukios E G
Bioresource Technology Unit, School of Chemical Engineering, National Technical University of Athens, Zografou Campus, Athens GR-15700, Greece.
Bioresour Technol. 2009 Dec;100(24):6331-8. doi: 10.1016/j.biortech.2009.07.011. Epub 2009 Aug 4.
The aim of this work was to evaluate the potential of employing biomass resources from different origin as feedstocks for fermentative hydrogen production. Mild-acid pretreated and hydrolysed barley straw (BS) and corn stalk (CS), hydrolysed barley grains (BG) and corn grains (CG), and sugar beet extract (SB) were comparatively evaluated for fermentative hydrogen production. Pretreatments and/or enzymatic hydrolysis led to 27, 37, 56, 74 and 45 g soluble sugars/100 g dry BS, CS, BG, CG and SB, respectively. A rapid test was applied to evaluate the fermentability of the hydrolysates and SB extract. The thermophilic bacterium Caldicellulosiruptor saccharolyticus showed high hydrogen production on hydrolysates of mild-acid pretreated BS, hydrolysates of BG and CG, and SB extract. Mild-acid pretreated CS showed limited fermentability, which was partially due to inhibitory products released in the hydrolysates, implying the need for the employment of a milder pretreatment method. The difference in the fermentability of BS and CS is in strong contrast to the similarity of the composition of these two feedstocks. The importance of performing fermentability tests to determine the suitability of a feedstock for hydrogen production was confirmed.
这项工作的目的是评估利用不同来源的生物质资源作为发酵产氢原料的潜力。对轻度酸预处理和水解的大麦秸秆（BS）、玉米秸秆（CS）、水解的大麦籽粒（BG）、玉米籽粒（CG）以及甜菜提取物（SB）进行了发酵产氢的比较评估。预处理和/或酶水解分别使每100克干BS、CS、BG、CG和SB产生27、37、56、74和45克可溶性糖。应用快速测试来评估水解产物和SB提取物的发酵能力。嗜热细菌嗜热解纤维梭菌在轻度酸预处理的BS水解产物、BG和CG水解产物以及SB提取物上表现出高产氢量。轻度酸预处理的CS显示出发酵能力有限，这部分是由于水解产物中释放出抑制性产物，这意味着需要采用更温和的预处理方法。BS和CS发酵能力的差异与这两种原料组成的相似性形成强烈对比。进行发酵能力测试以确定原料对产氢适用性的重要性得到了证实。
