Department of Biochemistry and Microbiology, "Paisii Hilendarski" University of Plovdiv, 24 Tzar Asen Str., 4000 Plovdiv, Bulgaria.
Bioelectrochemistry. 2010 Apr;78(1):57-61. doi: 10.1016/j.bioelechem.2009.07.005. Epub 2009 Jul 18.
Various prokaryote species have been widely studied for microbial fuel cell (MFC) application. However, the information about yeast utilization into biofuel cells is still scanty. The aim of this investigation is to verify if Candida melibiosica 2491, a yeast strain, possessing high phytase activity, could be applied as a biocatalyst in a yeast biofuel cell. The microbiological requirements were coupled with the electrochemical ones tracing main biochemical pathway metabolites such as different carbohydrate and inorganic phosphates and their assimilation with time. The obtained results show that from the three carbohydrates investigated - glucose, fructose and sucrose, fructose is the most suitable for the yeast cultivation. The presence of yeast extract and peptone improves the performance into the biofuel cell. The relationship between the yeast cell amount and the biofuel cell characteristics was determined. Analyses showed that electricity was generated by the yeast culture even in the absence of an artificial mediator. The addition of methylene blue at concentrations higher than 0.1 mM improves the current and power density output. The obtained experimental results proved that C. melibiosica 2491 belongs to the electrogenic strains.
已经有许多原核生物物种被广泛研究用于微生物燃料电池（MFC）应用。然而，关于酵母用于生物燃料电池的信息仍然很少。本研究的目的是验证具有高植酸酶活性的酵母菌株 Candida melibiosica 2491 是否可以作为生物催化剂应用于酵母生物燃料电池。微生物学要求与电化学要求相结合，追踪主要生化途径代谢物，如不同的碳水化合物和无机磷酸盐及其随时间的同化。获得的结果表明，在所研究的三种碳水化合物-葡萄糖、果糖和蔗糖中，果糖最适合酵母培养。酵母提取物和蛋白胨的存在提高了生物燃料电池的性能。确定了酵母细胞数量与生物燃料电池特性之间的关系。分析表明，即使没有人工介质，酵母培养也能产生电能。添加浓度高于 0.1mM 的亚甲蓝可以提高电流和功率密度输出。获得的实验结果证明 C. melibiosica 2491 属于发电菌株。
