Wiesak T, Przala J, Muszynska A, Hunter M G
Department of Animal Science, University of Alberta, Edmonton, Canada.
Endocrinol Exp. 1990 Dec;24(4):449-56.
Granulosa cells were recovered from small (1-3 mm in diameter) and large (greater than 6 mm in diameter) preovulatory follicles or from follicles of early pregnant pigs (3-5 mm, Day 18). Incubation of these cells (5 x 10(5)) was carried out in a shaking water bath (40 degrees C) for 2 h with or without salbutamol (10(-5) M), isoprenaline (10(-5) M), propranolol (10(-5) M) and FSH (100 ng/ml). Isoprenaline significantly increased progesterone production (P less than 0.05) by granulosa cells of small follicles and large preovulatory follicles but not by granulosa cells of follicles from early pregnant pigs. After blocking the beta-adrenoceptor with propranolol the stimulatory effect of catecholamines was not observed. FSH alone stimulated progesterone production, particularly in granulosa cells of early pregnant pigs (P less than 0.05) but FSH plus catecholamine treatment did not have any effect on progesterone release. These results suggest that catecholamines may play a regulatory role in follicle maturation and this may differ between naturally cyclic and early pregnant animals.
从小型（直径1 - 3毫米）和大型（直径大于6毫米）排卵前卵泡或妊娠早期母猪（3 - 5毫米，第18天）的卵泡中回收颗粒细胞。将这些细胞（5×10⁵）在振荡水浴（40℃）中孵育2小时，分别添加或不添加沙丁胺醇（10⁻⁵M）、异丙肾上腺素（10⁻⁵M）、普萘洛尔（10⁻⁵M）和促卵泡激素（FSH，100 ng/ml）。异丙肾上腺素显著增加了小型卵泡和大型排卵前卵泡颗粒细胞的孕酮分泌（P < 0.05），但对妊娠早期母猪卵泡的颗粒细胞没有影响。用普萘洛尔阻断β - 肾上腺素受体后，未观察到儿茶酚胺的刺激作用。单独使用FSH刺激孕酮分泌，特别是在妊娠早期母猪的颗粒细胞中（P < 0.05），但FSH加儿茶酚胺处理对孕酮释放没有任何影响。这些结果表明，儿茶酚胺可能在卵泡成熟中起调节作用，并且在自然发情周期动物和妊娠早期动物之间可能有所不同。
