去铁胺与超氧自由基反应的重新研究。脉冲辐解研究。

A reinvestigation of the reaction of desferrioxamine with superoxide radicals. A pulse radiolysis study.

作者信息

Goldstein S, Czapski G

机构信息

Department of Physical Chemistry, Hebrew University of Jerusalem, Israel.

出版信息

Free Radic Res Commun. 1990;11(4-5):231-40. doi: 10.3109/10715769009088920.

DOI:10.3109/10715769009088920

PMID:1965723

Abstract

The reaction of desferrioxamine with superoxide has been studied using the pulse radiolysis technique. The decay of O2- was not accelerated in the presence of up to 4 x 10(-4) M desferrioxamine at physiological pH. The rate constant was found to be lower than 2 x 10(4) M-1s-1. In acid solutions the rate constant of the reaction between desferrioxamine and HO'2 was found to be lower than 10(5) M-1s-1. The reaction was not studied in alkaline solutions due to the high absorbance of desferrioxamine in the U.V. region. The pK of desferrioxamine was determined to be 9.2 +/- 0.05.

摘要

已使用脉冲辐解技术研究了去铁胺与超氧化物的反应。在生理pH值下，存在高达4×10⁻⁴ M的去铁胺时，O₂⁻的衰减并未加速。发现速率常数低于2×10⁴ M⁻¹s⁻¹。在酸性溶液中，发现去铁胺与HO₂⁻之间反应的速率常数低于10⁵ M⁻¹s⁻¹。由于去铁胺在紫外区域的高吸光度，未在碱性溶液中研究该反应。测定出去铁胺的pK为9.2±0.05。