Portugal Raquel, Leitão Alexandre, Martins Carlos
Laboratório de Doenças Infecciosas, CIISA, Faculdade de Medicina Veterinária, Technical University of Lisbon, Av. da Universidade Técnica, 1300-477, Lisbon, Portugal.
Arch Virol. 2009;154(9):1441-50. doi: 10.1007/s00705-009-0466-x. Epub 2009 Aug 6.
African swine fever virus (ASFV) replicates in porcine macrophages. Since modulation of cell death by ASFV strains of different virulence is poorly understood, we studied the development of apoptosis in porcine macrophage cultures during in vitro infection with the high- and low-virulence isolates ASFV/L60 (L60) and ASFV/NH/P68 (NHV), respectively. In cultures inoculated with each isolate, similar numbers of cells hosting infection showed morphological signs of apoptosis, which were visible from a relatively early time of infection (8 h), although a significant proportion of the infected cell populations remained non-apoptotic until 18 h. L60 inhibited caspase-3 activation by 18 h after infection and induced less DNA internucleosomic fragmentation at 8 h than NHV. However, at the late infection time, apoptosis levels were similar in both infections and occurred, at least partially, independently of caspases, suggesting the existence of yet unknown alternative pathways committing ASFV host cells to apoptotic death.
非洲猪瘟病毒（ASFV）在猪巨噬细胞中复制。由于对不同毒力的ASFV毒株对细胞死亡的调节了解甚少，我们分别研究了高毒力分离株ASFV/L60（L60）和低毒力分离株ASFV/NH/P68（NHV）在体外感染猪巨噬细胞培养物过程中凋亡的发展情况。在接种每种分离株的培养物中，感染细胞的数量相似，都表现出凋亡的形态学特征，从感染相对早期（8小时）就可见，尽管在18小时之前，很大一部分感染细胞群体仍未凋亡。L60在感染后18小时抑制了caspase-3的激活，并且在8小时时比NHV诱导的DNA核小体间断裂更少。然而，在感染后期，两种感染中的凋亡水平相似，并且至少部分地独立于caspases发生，这表明存在尚未知的替代途径使ASFV宿主细胞走向凋亡死亡。
