Malendowicz L K, Dembińska M
Department of Histology and Embryology, Academy of Medicine, Poznań, Poland.
Folia Histochem Cytobiol. 1990;28(1-2):51-9.
The aim of the study was the analysis, by means of combined stereologic methods and metaphase arrest technique of the adrenocortical growth of ACTH treated hamster. Adult female hamsters were treated daily with 50 micrograms ACTH (Synacthen Depot, Ciba) for 3, 6 and 9 days. Between days 3 and 9 nearly linear increase in adrenal gland weight was observed, mainly due to the enlargement of the fasciculata and reticularis zones. Throughout the experiment, there was no marked change in the number of the zona glomerulosa cells while from the day 3 a notable increase in the number of fasciculata and reticularis cells occurred. Prolonged ACTH treatment resulted in a significant increase in 3H-thymidine incorporation with the highest value on day 6 and subsequent drop on day 9. Also metaphase index of adrenocortical cells notably increased due to ACTH administration and again the highest value was found on day 6 of experiment. From stereologic data and from metaphase index, adrenocortical cell birth-rates in individual adrenocortical zones and in the entire cortex were calculated. There were great differences between the values calculated from metaphase index and those obtained from stereologic data, with the former significantly lower. ACTH enhances centripetal migration of parenchymal cells in the hamster adrenal cortex and causes their accumulation in the zona reticularis.
本研究的目的是通过联合立体学方法和中期阻断技术，分析促肾上腺皮质激素（ACTH）处理的仓鼠肾上腺皮质的生长情况。成年雌性仓鼠每天接受50微克ACTH（辛纳科特长效注射剂，汽巴公司）处理，持续3天、6天和9天。在第3天至第9天期间，观察到肾上腺重量几乎呈线性增加，主要是由于束状带和网状带的增大。在整个实验过程中，球状带细胞数量没有明显变化，而从第3天起，束状带和网状带细胞数量显著增加。长期ACTH处理导致3H-胸腺嘧啶核苷掺入显著增加，在第6天达到最高值，随后在第9天下降。同样，由于ACTH给药，肾上腺皮质细胞的中期指数显著增加，在实验第6天再次达到最高值。根据立体学数据和中期指数，计算了各个肾上腺皮质区和整个皮质中的肾上腺皮质细胞出生率。从中期指数计算的值与从立体学数据获得的值之间存在很大差异，前者明显较低。ACTH增强了仓鼠肾上腺皮质实质细胞的向心迁移，并导致它们在网状带中积聚。
