Ueda Tomohiro, Manabe Hiroyuki, Tokuhiro Keizo, Hirose Mika, Matsuoka Yasuhiro, Miyagawa Yasushi, Tsujimura Akira, Fujita Kyoko, Wada Morimasa, Okuyama Akihiko, Nishimune Yoshitake, Tanaka Hiromitsu
Tanaka Project, Center for Advanced Science and Innovation, Osaka University, Suita, Osaka, Japan.
Int J Urol. 2009 Jul;16(7):639-46. doi: 10.1111/j.1442-2042.2009.02325.x.
To examine the expression profiles of the proteins translated from Acpin1 mRNA in germ cells.
Northern and western blotting of various tissues and immunohistochemical analysis of germ cells were carried out in a mouse model.
ACPIN1 protein was transcribed from the longer, 3' open reading frame (ORF) of Acpin1. An alternative-splicing variant, Acpin1vs, contained only the smaller, 5' ORF of the full-length Acpin1 gene. Its gene product, SAGSIN1, was expressed specifically in salivary glands. Retrotransposed regions of Acpin1 homology were also detected in various chromosomes, and intronless paralogous genes on the X chromosome were expressed in the testis and other tissues. The genomic structure of Acpin1 is highly conserved in mammals.
The two ORFs on the Acpin1 mRNA are independently translated in differentiated cells. Analysis of gene Acpin1 might clarify the molecular mechanism of spermatogenesis.
检测从Acpin1 mRNA翻译而来的蛋白质在生殖细胞中的表达谱。
在小鼠模型中对各种组织进行Northern和western印迹分析，并对生殖细胞进行免疫组织化学分析。
ACPIN1蛋白由Acpin1较长的3'开放阅读框（ORF）转录而来。一种可变剪接变体Acpin1vs仅包含全长Acpin1基因较小的5' ORF。其基因产物SAGSIN1在唾液腺中特异性表达。在各种染色体中也检测到了Acpin1同源性的反转座区域，X染色体上的无内含子旁系同源基因在睾丸和其他组织中表达。Acpin1的基因组结构在哺乳动物中高度保守。
Acpin1 mRNA上的两个ORF在分化细胞中独立翻译。对Acpin1基因的分析可能会阐明精子发生的分子机制。
