Pradier P, Tournaire C, Dalle M
Laboratoire de Physiologie Animale, Université Blaise Pascal Clermont II, Aubière, France.
J Dev Physiol. 1990 Sep;14(3):163-9.
Comparison of effects of synthetic ovine corticotropin releasing factor (oCRF), Arginine-Vasopressin (AVP) and the combination of both peptides have been tried in adult and 7-days-old guinea-pigs. On plasmas collected 15 min after interscapulary injection, cortisol, aldosterone and ACTH were measured. The different circulating forms of ACTH were isolated by Sephadex G50 column chromatography, with 1% formic acid and measured by radioimmunoassay. Thus, in the guinea-pig plasma, we detected three immunoreactive forms of ACTH: a "big" molecular form (Mr greater than 20000), an "intermediate" (Mr = 9500) and a "little" ACTH form (Mr = 4500) which was eluted in the same fractions as human 1-39 ACTH. In adult guinea-pigs, CRF increased total ACTH and the "intermediate" form and also plasma cortisol concentrations whereas AVP remained without significant effect excepted a rise in cortisol levels. Injected together, CRF and AVP enhanced plasma concentrations of total ACTH, of the three circulating forms and of cortisol. In 7-days-old guinea-pigs, both CRF and AVP increased plasma concentrations of total, of "intermediate" ACTH and of cortisol and aldosterone whereas the combination of both peptides enhanced dramatically plasma concentration of total ACTH suggesting a magnifying effect of AVP on CRF activity still more efficient in young than in adult guinea-pigs.
已在成年和7日龄豚鼠中尝试比较合成羊促肾上腺皮质激素释放因子（oCRF）、精氨酸加压素（AVP）以及这两种肽组合的作用。在肩胛间注射后15分钟采集的血浆中，测量皮质醇、醛固酮和促肾上腺皮质激素（ACTH）。通过Sephadex G50柱色谱法，用1%甲酸分离ACTH的不同循环形式，并通过放射免疫测定法进行测量。因此，在豚鼠血浆中，我们检测到ACTH的三种免疫反应形式：一种“大”分子形式（分子量大于20000）、一种“中间”形式（分子量 = 9500）和一种“小”ACTH形式（分子量 = 4500），其与人类1 - 39 ACTH在相同级分中洗脱。在成年豚鼠中，CRF增加了总ACTH和“中间”形式以及血浆皮质醇浓度，而AVP除了使皮质醇水平升高外没有显著影响。CRF和AVP一起注射时，增强了总ACTH、三种循环形式以及皮质醇的血浆浓度。在7日龄豚鼠中，CRF和AVP都增加了总ACTH、“中间”ACTH以及皮质醇和醛固酮的血浆浓度，而两种肽的组合显著提高了总ACTH的血浆浓度，表明AVP对CRF活性的放大作用在幼龄豚鼠中比成年豚鼠中更有效。
