Kownatzki E, Uhrich S
Department of Dermatology, University of Freiburg, FRG.
Int Arch Allergy Appl Immunol. 1990;93(4):344-9. doi: 10.1159/000235264.
During the chemotactic migration of human neutrophilic granulocytes towards the chemotactic factors f-Met-Leu-Phe, C5a, leukotriene B4 (LTB4), monocyte-derived chemotaxin (MOC/IL-8) and platelet-activating factor (PAF) in Boyden chambers, the production of superoxide anion and hydrogen peroxide was measured by superoxide dismutase-inhibitable cytochrome C reduction and oxidation of p-OH-phenylacetic acid, respectively. With the exception of 10(-6) M PAF, none of the factors at optimal chemotactic concentrations induced the production of O2- or H2O2 in amounts significantly different from neutrophilic granulocytes migrating at random. At 20-50 times the optimal chemotactic concentration some O2- and H2O2 production was observed with f-Met-Leu-Phe, C5a and LTB4, but not with MOC/IL-8. Superoxide dismutase, catalase or a combination of the two added to both compartments of the Boyden chambers did not affect the random or chemotactic migration towards any of the chemotactic factors. The results suggest that chemotactic migration and the production of reactive oxygen metabolites by human neutrophilic granulocytes are unrelated events.
在博伊登小室中，人嗜中性粒细胞向趋化因子N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸（f-Met-Leu-Phe）、C5a、白三烯B4（LTB4）、单核细胞衍生趋化因子（MOC/IL-8）和血小板活化因子（PAF）进行趋化迁移的过程中，分别通过超氧化物歧化酶抑制的细胞色素C还原法和对羟基苯乙酸氧化法来测定超氧阴离子和过氧化氢的产生量。除了10⁻⁶ M的PAF外，在最佳趋化浓度下，没有一种因子能诱导产生与随机迁移的嗜中性粒细胞有显著差异的超氧阴离子或过氧化氢量。在最佳趋化浓度的20至50倍时，f-Met-Leu-Phe、C5a和LTB4可观察到一些超氧阴离子和过氧化氢的产生，但MOC/IL-8则没有。将超氧化物歧化酶、过氧化氢酶或两者的组合添加到博伊登小室的两个隔室中，并不影响对任何趋化因子的随机或趋化迁移。结果表明，人嗜中性粒细胞的趋化迁移与活性氧代谢产物的产生是不相关的事件。
