Smith R J, Capaldi R A
Biochemistry. 1977 Jun 14;16(12):2629-33. doi: 10.1021/bi00631a008.
Ubiquinone cytochrome c reductase (complex III) in detergent dispersion has been cross-linked with two reversible cross-linking agents dithiobissuccinimidylpropionate and dimethyl-3.3'-dithiobispropionimidate and the cross-linked products formed have been analyzed by two-dimensional gel electrophoresis. Under mild reaction conditions, polypeptides I and II, II and VI, I and V, and VI and VII were the most prominent subunit pairs seen. With higher levels of reagent, larger aggregates were produced until an aggregate of apparent molecular weight 310 000 was the dominant band on gels. This is the complex III monomer.
去污剂分散体系中的泛醌细胞色素c还原酶（复合体III）已用两种可逆交联剂二硫代双琥珀酰亚胺丙酸酯和二甲基-3,3'-二硫代双丙亚胺酸酯进行交联，并且通过二维凝胶电泳分析了所形成的交联产物。在温和的反应条件下，多肽I和II、II和VI、I和V以及VI和VII是最明显可见的亚基对。使用更高水平的试剂时，会产生更大的聚集体，直到表观分子量为310000的聚集体成为凝胶上的主要条带。这就是复合体III单体。
