Wilson D R, Chatfield S, Betts J, Griffiths A, Leung K Y, Dougan G, Finlay B B
Biotechnology Laboratory, University of British Columbia, Vancouver, Canada.
Res Microbiol. 1990 Sep-Oct;141(7-8):827-30. doi: 10.1016/0923-2508(90)90117-9.
Because of their invasive properties, Salmonella species are uniquely suited for use as live oral vaccines, and mutants attenuated in general metabolic functions show promise as vaccine strains. We have identified a Salmonella choleraesuis transposon mutant which is deficient in abilities to attach to, invade or penetrate through animal cells. This mutant is avirulent in mice, yet is able to persist within the liver, spleen and gut-associated lymphoid tissue for periods which may be adequate for induction of secretory, humoral and cell-mediated immunity. This and other strains attenuated in factors specifically required for Salmonella pathogenesis may have potential as vaccine strains.
由于具有侵袭特性，沙门氏菌特别适合用作口服活疫苗，而在一般代谢功能上减毒的突变体有望成为疫苗菌株。我们鉴定出一株猪霍乱沙门氏菌转座子突变体，它在附着、侵袭或穿透动物细胞的能力方面存在缺陷。该突变体对小鼠无毒力，但能够在肝脏、脾脏和肠道相关淋巴组织中持续存在一段时间，这段时间可能足以诱导分泌性、体液性和细胞介导的免疫。这种以及其他在沙门氏菌致病所需特定因子上减毒的菌株可能具有作为疫苗菌株的潜力。
