Li D, Cavanagh D
AFRC Institute for Animal Health, Houghton Laboratory, Huntingdon, Cambridgeshire, UK.
Adv Exp Med Biol. 1990;276:33-6. doi: 10.1007/978-1-4684-5823-7_5.
Syncytium formation induced in Vero cells by IBV-Beaudette was optimal at pH 6.7 and absent at pH 7.5. Reduction of IBV-Beaudette replication by ammonium chloride, which raises the pH in endosomes, further indicated that uncoating of the virus genome probably occurred within the acidic environment of endosomes. However, this would not appear to apply to all IBV strains, since one was unaffected by the drug. It would appear that some strains of IBV have the capacity to fuse with the plasma membrane at neutral pH, thereby leading to genome uncoating at that site. Other strains of IBV might also uncoat at the plasma membrane when the extracellular environment is slightly less than pH7.
传染性支气管炎病毒-Beaudette株（IBV-Beaudette）在Vero细胞中诱导形成合胞体的最佳pH值为6.7，在pH 7.5时则不会形成。氯化铵可提高内体的pH值，从而减少IBV-Beaudette的复制，这进一步表明病毒基因组的脱壳可能发生在内体的酸性环境中。然而，这似乎并不适用于所有IBV毒株，因为有一种毒株不受该药物影响。看来一些IBV毒株有能力在中性pH值下与质膜融合，从而导致在该部位发生基因组脱壳。当细胞外环境的pH值略低于7时，其他IBV毒株也可能在质膜处脱壳。
