Hu Ye-Rong, Ren Hong, Liu Zhe-Liang, Yu Feng-Lei, Liu Wen-Liang, Zhou Xin-Min
Department of Cardiothoracic Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, P. R. China.
Ai Zheng. 2009 Aug;28(8):890-3. doi: 10.5732/cjc.009.10019.
Targeted therapies have become a valuable therapeutic option for cancer. Establishment of different animal tumor models has become necessary. This study was to establish xenotransplantation models for patient-derived non-small cell lung cancer (NSCLC) in immune deficient mice.
Immune deficient mice, BALB/C-nu, NOD/scid and SCID, 16 in each strain, were used. Sixteen tumor specimens were obtained from patients with NSCLC. Each specimen was subcutaneously transplanted into one mouse from each of the three strains. The tumor formation rate, time to tumor engraftment, tumor volume doubling time were recorded and compared among the three strains of mice. Histology of xenograft tumors was examined.
The total tumor formation rate was 75% (12/16). The tumor formation rate was the highest in SCID mice (56.25%). Only four tumors were engrafted in SCID mice, and two in BALB/C-nu mice. The tumor formation rate, time to tumor engraftment, and tumor volume doubling time were not significantly different among the three strains of mice. The incidence of tumor size over 1cm in the upper flanks of the mice (56.25%) was significantly higher than that in the lower flanks (25%) (P=0.037). Haematoxylin Eosin staining revealed a high degree of histological similarity between all xenograft and the parental tumors.
We have established xenotransplantation models for patient-derived NSCLC with a success rate of 75% in BALB/C-nu and SCID mice. The xenograft tumors have the same histological features to those of their parental tumors.
靶向治疗已成为癌症治疗的重要选择。建立不同的动物肿瘤模型变得十分必要。本研究旨在建立免疫缺陷小鼠的人源非小细胞肺癌（NSCLC）异种移植模型。
使用免疫缺陷小鼠，BALB/C-nu、NOD/scid和SCID各16只。从NSCLC患者获取16个肿瘤标本。每个标本分别皮下移植到三种品系的各一只小鼠体内。记录并比较三种品系小鼠的肿瘤形成率、肿瘤接种时间、肿瘤体积倍增时间。检查异种移植肿瘤的组织学情况。
总肿瘤形成率为75%（12/16）。SCID小鼠的肿瘤形成率最高（56.25%）。SCID小鼠仅接种了4个肿瘤，BALB/C-nu小鼠接种了2个。三种品系小鼠的肿瘤形成率、肿瘤接种时间和肿瘤体积倍增时间无显著差异。小鼠上腹部肿瘤大小超过1cm的发生率（56.25%）显著高于下腹部（25%）（P=0.037）。苏木精-伊红染色显示所有异种移植肿瘤与其亲本肿瘤在组织学上高度相似。
我们成功建立了人源NSCLC异种移植模型，在BALB/C-nu和SCID小鼠中的成功率为75%。异种移植肿瘤与其亲本肿瘤具有相同的组织学特征。
