AminoScience Laboratories, Ajinomoto Co., Inc., 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki 210-8681, Japan.
J Biosci Bioeng. 2009 Sep;108(3):190-3. doi: 10.1016/j.jbiosc.2009.03.018.
Screening was carried out for microorganisms able to produce N-(l-alpha-l-aspartyl)-l-phenylalanine methyl ester [APM] from l-isoasparagine and l-phenylalanine methyl ester hydrochloride. Of the 422 strains examined, 44 strains belonging to the family Enterobacteriaceae were found to produce APM. The enzyme catalyzing APM production was purified and identified as dipeptidase E.
进行了筛选能够从 l-异天冬酰胺和 l-苯丙氨酸甲酯盐酸盐生产 N-(l-α-l-天冬氨酰基)-l-苯丙氨酸甲酯 [APM] 的微生物的实验。在检查的 422 株菌株中，发现有 44 株属于肠杆菌科的菌株能够生产 APM。催化 APM 生产的酶被纯化并鉴定为二肽酶 E。
