Chin Ji-Hyang, Shiwaku Hiroki, Goda Olga, Komuro Akihiko, Okazawa Hitoshi
Department of Neuropathology, Medical Research Institute, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.
Biochem Biophys Res Commun. 2009 Oct 16;388(2):247-51. doi: 10.1016/j.bbrc.2009.07.165. Epub 2009 Aug 5.
There remain controversy and disagreement on whether Oct-3/4 is expressed in neural stem cells or not. Although many reports had shown expression of Oct-3/4 in somatic stem and progenitor cells, conditional KO mice of Oct-3/4 in neural stem cells did not show any phenotype. Even pseudogenes are suspected for the "false positive" results. However, we here report that Oct-3/4 but not pseudogenes is actually expressed in neural stem cells. Western blot analysis with multiple Oct-3/4 antibodies also showed protein expression of Oct-3/4. The subnuclear localization of Oct-3/4 is clearly different from that of P19 cells, suggesting that Oct-3/4 might be inactivated by other mechanisms than transcriptional repression.
关于Oct-3/4是否在神经干细胞中表达仍存在争议和分歧。尽管许多报告显示Oct-3/4在体干细胞和祖细胞中有表达，但神经干细胞中Oct-3/4的条件性敲除小鼠并未表现出任何表型。甚至有人怀疑假基因导致了“假阳性”结果。然而，我们在此报告Oct-3/4而非假基因实际上在神经干细胞中表达。用多种Oct-3/4抗体进行的蛋白质印迹分析也显示了Oct-3/4的蛋白表达。Oct-3/4的亚核定位与P19细胞明显不同，这表明Oct-3/4可能通过转录抑制以外的其他机制失活。
