Mössner R, Sedgwick J, Flory E, Körner H, Wege H, ter Meulen V
Institute for Virology and Immunobiology, University of Würzburg, West Germany.
Adv Exp Med Biol. 1990;276:647-54. doi: 10.1007/978-1-4684-5823-7_88.
CD4+ T cell lines specific for murine hepatitis virus (MHV) - JHM or myelin basic protein (MBP) proliferated when cultured together with MHC class I and II positive syngeneic rat astrocytes and either inactivated virus or MBP as antigen. The magnitude of the T cell proliferative response was comparable to that seen when thymocytes were used as a source of antigen presenting cells (APC). In contrast, MHC class I and II positive astrocytes were unable to significantly stimulate the proliferation of highly purified populations of naive CD4+ and CD8+ T cells in an allogeneic mixed lymphocyte reaction (MLR). Both T cell populations proliferated when mixed with allogeneic lymph node cells. Infection of the astrocytes with a variant of MHV-JHM (PI-AS22D) did not alter this cells incapacity to stimulate the naive CD4+ and CD8+ T cells to proliferate.
当与I类和II类主要组织相容性复合体（MHC）阳性的同基因大鼠星形胶质细胞以及作为抗原的灭活病毒或髓鞘碱性蛋白（MBP）一起培养时，对鼠肝炎病毒（MHV）-JHM或髓鞘碱性蛋白（MBP）具有特异性的CD4 + T细胞系会增殖。T细胞增殖反应的强度与以胸腺细胞作为抗原呈递细胞（APC）来源时所见的强度相当。相比之下，在同种异体混合淋巴细胞反应（MLR）中，I类和II类MHC阳性星形胶质细胞无法显著刺激高度纯化的幼稚CD4 +和CD8 + T细胞群体的增殖。当与同种异体淋巴结细胞混合时，两个T细胞群体都会增殖。用MHV-JHM变体（PI-AS22D）感染星形胶质细胞并不会改变这种细胞无法刺激幼稚CD4 +和CD8 + T细胞增殖的情况。
