Kato Yasuhiro X, Saito Itsuro, Takano Haruka, Mabuchi Kunihiko, Hoshino Takayuki
NTT Communication Science Laboratories, Nippon Telegraph and Telephone Corporation, 243-0198 Kanagawa, Japan.
Neurosci Lett. 2009 Oct 16;464(1):26-8. doi: 10.1016/j.neulet.2009.08.007. Epub 2009 Aug 5.
DiX C (or Parylene-C) has been widely used as a coating material to insulate neural electrodes in recent decades. However, its uses are limited due to its extremely low adhesiveness with neuronal cells. Other functional materials in the diX family, such as diX A, diX AM, and diX H, have been commercialized recently and would offer different features in biocompatibility from diX C. However, their cell adhesiveness remains unknown. In this work, we used an in vitro approach to investigate how the surface of each material in the diX family affects the degree of neuronal cell adhesiveness compared with a conventional culture dish of polystyrene (PS). The neuronal cell adhesiveness on diX AM and diX H was almost equivalent to that for the PS dish, whereas neuronal cells did not settle on the surface of diX C and diX A. Our results suggest that diX AM and diX H could provide another practical feature as a coating material for a scaffold in a substrate with any configuration in neural devices.
近几十年来，聚对二甲苯C（或派瑞林C）作为一种用于绝缘神经电极的涂层材料被广泛使用。然而，由于其与神经元细胞的粘附性极低，其应用受到限制。聚对二甲苯家族中的其他功能材料，如聚对二甲苯A、聚对二甲苯AM和聚对二甲苯H，最近已商业化，并且在生物相容性方面将提供与聚对二甲苯C不同的特性。然而，它们的细胞粘附性仍然未知。在这项工作中，我们采用体外方法研究了聚对二甲苯家族中每种材料的表面与传统聚苯乙烯（PS）培养皿相比如何影响神经元细胞的粘附程度。聚对二甲苯AM和聚对二甲苯H上的神经元细胞粘附性几乎与PS培养皿上的相当，而神经元细胞不会在聚对二甲苯C和聚对二甲苯A的表面沉降。我们的结果表明，聚对二甲苯AM和聚对二甲苯H作为神经装置中任何构型的基底支架的涂层材料可以提供另一个实用特性。
