Hou Qiao-Fang, Li Sheng-Bin
Key Laboratory of the National Ministry of Health for Forensic Sciences, College of Medicine, Xi'an Jiaotong University, 76 Yanta West Road, Xi'an 710061, PR China.
Neurosci Lett. 2009 Oct 23;464(2):127-30. doi: 10.1016/j.neulet.2009.08.004. Epub 2009 Aug 5.
The aim of our study was to investigate the potential association of dopamine receptor D2 gene (DRD2) TaqI RFLP A (rs1800497) and dopamine transporter gene (DAT) 3'untranslated region VNTR genetic variations with heroin addiction. Genotyping was performed using PCR-based techniques in 530 heroin abusers and 500 controls. Our results showed that DRD2 TaqI A1 allele carriers (genotypes A1A1 and A1A2) were prone to heroin abuse in models of dominance or co-dominance. We detected a 12 repeat allele and 6/6, 7/9, 9/11, 10/12 genotype in a Chinese/eastern Asian population for the first time. However, no significant differences in the DAT1 VNTR were found between the two groups in either genotypic or allelic distributions and there was no gene interaction between the two genetic loci.
我们研究的目的是调查多巴胺受体D2基因（DRD2）TaqI限制性片段长度多态性A（rs1800497）和多巴胺转运体基因（DAT）3'非翻译区可变数目串联重复序列的基因变异与海洛因成瘾之间的潜在关联。采用基于聚合酶链反应的技术对530名海洛因滥用者和500名对照者进行基因分型。我们的结果显示，在显性或共显性模型中，DRD2 TaqI A1等位基因携带者（基因型A1A1和A1A2）易于滥用海洛因。我们首次在中国/东亚人群中检测到12重复等位基因以及6/6、7/9、9/11、10/12基因型。然而，两组之间在DAT1可变数目串联重复序列的基因型或等位基因分布上均未发现显著差异，并且两个基因位点之间不存在基因相互作用。
